B-hIL4/IL4RA mice

Basic Information

Strain Name
C57BL/6-Il4tm2(IL4)Il4ratm1(IL4RA)/Bcgen
Common Name
B-hIL4/hIL4RA  mice
Background strain
C57BL/6
Catalog Number
120551
Related Genes
IL4: interleukin 4; IL4RA: interleukin 4 receptor, IL4R

Description

IL4 (interleukin 4) encoded by this gene is a pleiotropic cytokine produced by activated T cells. IL4 is capable of binding to its receptor IL4R, while IL13 is also capable of binding to IL4R, which may result in a functional overlap of IL4 and IL13. IL-4 has immunomodulatory effects on B cells, T cells, mast cells, macrophages, and the like. It can promote the proliferation of antigen or mitogen-activated B cells; promote T cell activity, single IL-4 can not stimulate macrophage proliferation, but can enhance macrophage function.

IL4R (interleukin 4 receptor) encodes the alpha chain of the IL4 receptor and belongs to the type I transmembrane protein, which plays a huge role in the immune response. IL4R can bind to IL4 and IL13 to regulate the production of IgE, and binding to IL4 can promote the differentiation of Th2 cells, thereby inducing the occurrence of asthma.

Therefore, antagonists of IL4 and IL4R can reduce the level of IL4 in the body, and can exert anti-inflammatory and anti-immune effects on asthma patients. IL4 and IL4R are considered to be star targets for the treatment of allergies and asthma.

Details

Protein expression analysis

Strain specific IL4 expression analysis in homozygous B-hIL4/hIL4RA mice by ELISA.

Serum were collected from WT and homozygous B-hIL4/hIL4RA (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by ELISA with species-specific IL4 ELISA kit. Mouse Il4 was detectable in WT mice. Human IL4 was exclusively detectable in homozygous B-hIL4/hIL4RA  mice but not WT mice.

 

Strain specific IL4RA expression analysis in homozygous B-hIL4/hIL4RA mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hIL4/hIL4RA (H/H) mice, and analyzed by flow cytometry with species-specific anti-IL4RA antibody. Mouse Il4ra was detectable in WT mice. Human IL4RA was exclusively detectable in homozygous B-hIL4/hIL4RA   mice but not WT mice.

Phenotypic analysis 

Analysis of spleen leukocytes cell subpopulations in B-hIL4/hIL4RA mice

Analysis of spleen leukocyte subpopulations by FACS

Splenocytes were isolated from female C57BL/6 and B-hIL4/hIL4RA mice (n=3, 10-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45 population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T, B, NK, Monocyte, DC and macrophage cells in homozygous B-hIL4/hIL4RA  mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL4 and hIL4RA in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen.

Analysis of spleen T cell subpopulations in B-hIL4/hIL4RA mice

Analysis of spleen T cell subpopulations by FACS

Splenocytes were isolated from female C57BL/6 and B-hIL4/hIL4RA mice (n=3, 10-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ T cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8, CD4, and Treg cells in homozygous B-hIL4/hIL4RA mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL4 and hIL4RA in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell sub types in spleen. Values are expressed as mean ± SEM.

Analysis of Lymph node leukocyte subpopulations in B-hIL4/hIL4RA mice

Analysis of subpopulation of leukocytes in lymph node by FACS

Lymph node were isolated from female C57BL/6 and B-hIL4/hIL4RA mice (n=3, 10-week-old). Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ T cells were used for further analysis as indicated here. B. Results of FACS analysis. Percent of T, B, and NK cells in homozygous B-hIL4/hIL4RA  mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL4 and hIL4RA in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.

Analysis of lymph node T cell subpopulations in B-hIL4/hIL4RA mice

Analysis of subpopulation of T cells in lymph node by FACS

Lymph node were isolated from female C57BL/6 and B-hIL4/hIL4RA mice (n=3, 10-week-old). Flow cytometry analysis of the lymph node was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ T cells were used for further analysis as indicated here. B. Results of FACS analysis. Percent of CD8, CD4, and Treg cells in homozygous B-hIL4/hIL4RA  mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hIL4 and hIL4RA in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in lymph node. Values are expressed as mean ± SEM.

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