Basic Information

Strain Name
C57BL/6-Il5tm1(IL5)Il5ratm2(IL5RA)/Bcgen
Common Name
B-hIL5/hIL5RA mice
Background
C57BL/6N
Catalog number
111932
Related Genes
EDF, IL-5, TRF; CD125, CDw125, HSIL5R3, IL5R
NCBI Gene ID
IL5: 3567; IL5RA: 3568

Protein expression analysis

Species-specific IL5 protein expression analysis in wild-type and humanized B-hIL5 mice. Following ConA stimulation in vivo, serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hIL5 (H/H) mice and analyzed by ELISA using species-specific IL5 kits. Murine IL5 protein was detected in wild-type mice, while human IL5 protein was detected in B-hIL5 mice.

Species-specific IL5RA protein expression analysis in wild-type and humanized B-hIL5/hIL5RA mice. Blood-derived eosinophils were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hIL5/hIL5RA (H/+) mice and analyzed by flow cytometry using species-specific anti-IL5RA antibodies. Murine IL5RA protein was detected in wild-type and heterozygous mice, while human IL5RA protein was only detected in heterozygous B-hIL5/hIL5RA mice.