Basic Information
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Protein expression analysis
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Species-specific IL5 protein expression analysis in wild-type and humanized B-hIL5 mice. Following ConA stimulation in vivo, serum was collected from wild-type C57BL/6 (+/+) and homozygous B-hIL5 (H/H) mice and analyzed by ELISA using species-specific IL5 kits. Murine IL5 protein was detected in wild-type mice, while human IL5 protein was detected in B-hIL5 mice.
Species-specific IL5RA protein expression analysis in wild-type and humanized B-hIL5/hIL5RA mice. Blood-derived eosinophils were isolated from wild-type C57BL/6 (+/+) and heterozygous B-hIL5/hIL5RA (H/+) mice and analyzed by flow cytometry using species-specific anti-IL5RA antibodies. Murine IL5RA protein was detected in wild-type and heterozygous mice, while human IL5RA protein was only detected in heterozygous B-hIL5/hIL5RA mice.
