Basic Information
Strain Name
C57BL/6-Magtm1(MAG)/Bcgen
Common Name
B-hMAG mice
Background
C57BL/6N
Catalog number
111033
Aliases
MAG (GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A)
NCBI Gene ID
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Gene targeting strategy
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Gene targeting strategy for B-hMAG mice. The exons 4-9 of mouse MAG gene that encode the extracellular domain were replaced by human MAG exons 3-8 in B-hMAG mice.
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mRNA expression analysis
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Species-specific MAG gene expression analysis in wild-type and humanized B-hMAG mice by RT-PCR. Murine Mag mRNA was detected in brain tissue isolated from wild-type C57BL/6 (+/+) mice, while human MAG mRNA was detected in homozygous B-hMAG (H/H) mice.
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Protein expression analysis
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Species-specific MAG protein expression analysis in wild-type and humanized B-hMAG mice. Brain tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hMAG (H/H) mice and analyzed by ELISA using species-specific anti-MAG kits. Due to the cross-reactive antibodies, MAG protein was detected in wild-type and B-hMAG mice.
