MAG (GMA; S-MAG; SPG75; SIGLEC4A; SIGLEC-4A)
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hMAG mice. The exons 4-9 of mouse MAG gene that encode the extracellular domain were replaced by human MAG exons 3-8 in B-hMAG mice.
mRNA expression analysis
Species-specific MAG gene expression analysis in wild-type and humanized B-hMAG mice by RT-PCR. Murine Mag mRNA was detected in brain tissue isolated from wild-type C57BL/6 (+/+) mice, while human MAG mRNA was detected in homozygous B-hMAG (H/H) mice.
Protein expression analysis
Species-specific MAG protein expression analysis in wild-type and humanized B-hMAG mice. Brain tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hMAG (H/H) mice and analyzed by ELISA using species-specific anti-MAG kits. Due to the cross-reactive antibodies, MAG protein was detected in wild-type and B-hMAG mice.