Basic Information

Strain name
C57BL/6-Tslptm1(TSLP)Crlf2tm2(CRLF2)/Bcgen
Common name
B-hTSLP/hTSLPR mice
Catalog number
121269
Background
C57BL/6
Aliases
CRL2Y, TSLPR, CRLF2
NCBI Gene ID

Gene Targeting Strategy

Gene targeting strategy for B-hTSLP/hTSLPR mice. The exons 1-5 of mouse Tslp gene that encode the full-length protein were replaced by human TSLP exons 1-4 in B-hTSLP/hTSLPR mice. The extracellular and transmembrane region of human TSLPR gene and the cytoplasmic region of mouse Tslpr gene were constructed into a chimeric CDS vector and inserted into the mouse exon 2. The targeted mice will express the chimeric TSLPR protein, while mouse TSLPR will no longer express.

TSLP Protein Expression Analysis

Species-specific TSLP expression analysis in heterozygous B-hTSLP mice by ELISA. Calcipotriol (MC903) was dissolved in ethanol and topically applied on ears of either wild-type C57BL/6 (+/+) or heterozygous B-hTSLP mice (H/+) for 3 days. Ear grinding supernatant was analyzed by ELISA. Murine TSLP protein was detected in wild-type and heterozygous B-hTSLP mice, while human TSLP was exclusively detected in heterozygous B-hTSLP mice.

TSLPR mRNA Expression Analysis

Species-specific TSLPR gene expression analysis in wild type and heterozygous B-hTSLP/hTSLPR mice. Murine Tslpr mRNA was detected in splenocytes isolated from wild-type C57BL/6 (+/+) and heterozygous B-hTSLP/hTSLPR (H/+;H/+) mice. Human TSLPR mRNA was exclusively detected in humanized B-hTSLP/hTSLPR mice.

TSLPR Protein Expression Analysis

Species-specific TSLPR expression analysis in heterozygous B-hTSLP/hTSLPR mice. Macrophages were collected from wild-type C57BL/6 (+/+) and heterozygous B-hTSLP/hTSLPR (H/+;H/+) mice, and analyzed by flow cytometry using species-specific anti-TSLPR antibodies. Murine TSLPR protein was detected in wild-type and heterozygous B-hTSLP/hTSLPR mice, while human TSLPR protein was exclusively detected in humanized B-hTSLP/hTSLPR mice.

 

Species-specific TSLPR expression analysis in heterozygous B-hTSLP/hTSLPR mice. Following LPS stimulation, dendritic cells isolated from the bone marrow of wild-type C57BL/6 (+/+) and heterozygous B-hTSLP/hTSLPR (H/+) mice were analyzed by flow cytometry using species-specific anti-TSLPR antibodies. Murine TSLPR protein was detected in wild-type and heterozygous B-hTSLP/hTSLPR mice, while human TSLPR protein was exclusively detected in humanized B-hTSLP/hTSLPR mice.

Poster

Functional Analysis of Anti-TSLP Antibodies Using Humanized B-hTSLP/hTSLPR Mice