Basic Information

Strain Name
C57BL/6-Vegfatm1(VEGFA)/Bcgen
Common Name
B-hVEGFA mice
Background
C57BL/6
Catalog number
110822
Related Genes
VEGFA (MVCD1, VEGF, VPF)
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hVEGFA mice.

The exons 1-8 of mouse Vegfa gene that encode the full-length protein were replaced by human VEGFA exons 1-8 in B-hVEGFA mice.

Protein expression analysis

Strain specific VEGFA expression analysis in homozygous B-hVEGFA mice by ELISA. Mouse lung homogenates was collected from wild-type mice (+/+) and B-hVEGFA mice (H/H), and analyzed by ELISA with species-specific VEGFA kit. Mouse VEGFA was detectable in wild-type mice. Human VEGFA was exclusively detectable in B-hVEGFA mice but not in wild-type mice.

Fundus photographs of B-hVEGFA mice

Fundus photographs of B-hVEGFA mice. Fundus images shows that the  fundus morphology of B-hVEGFA mice has no significant change compared with wild-type mice (male, n=3).

Angiogenesis analysis

We investigated by immunohistochemistry the expression of mCD31 in the heart, liver, lung, kidney and eye of C57BL/6 mice and B-hVEGFA mice (female, 8 week-old, n=3). The results showed that there was no significant difference in the proportion of mCD31+ between C57BL/6 mice and B-hVEGFA mice in heart, liver, lung, kidney, eye. It is suggested that VEGFA humanized has no effect on angiogenesis.

Summary

Protein expression analysis:

Human VEGFA was exclusively detectable in lung homogenates of B-hVEGFA mice but not in wild-type mice.

Fundus photographs of B-hVEGFA mice

Fundus images show the  fundus morphology of B-hVEGFA mice showed no significant changes compared with wild-type mice.

Angiogenesis analysis

There was no significant difference in the proportion of mCD31+ between C57BL/6 mice and B-hVEGFA mice in heart, liver, lung, kidney, eye. It is suggested that VEGFA humanized has no effect on angiogenesis.