Basic Information
Strain Name
C57BL/6-Glp1rtm1(GLP1R)/Bcgen
Stock Number
170164
Common Name
B-hGLP1R mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
GLP-1; GLP-1R; GLP-1-R
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous
NCBI Gene ID
-
Target strategy
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The full coding sequence of the human GLP1R gene was inserted into mouse Glp1r gene in B-hGLP1R mice.
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mRNA and protein expression analysis
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RT-PCR and western blot analysis of GLP1R. The mRNA of human GLP1R was detectable in the lung of the homozygous B-hGLP1R mice, but not detectable in wildtype mice. and we proved that the protein could be expressed normally by western blot analysis. Human and mouse cross-reactive antibodies were used to detect the GLP1R of the wildtype and humanized GLP1R mice.
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Protein expression analysis
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IHC for human GLP1R expression. Pancreata from wild-type (+/+) and B-hGLP1R mice (H/H) mice were stained using an antibody specific for human GLP1R. and only the pancreas from B-hGLP1R mice (H/H) mice showed hGLP1R positive signal in plasma membrane.
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Poster