The full coding sequence of the human GLP1R gene was inserted into mouse Glp1r gene in B-hGLP1R mice.
mRNA and protein expression analysis
RT-PCR and western blot analysis of GLP1R. The mRNA of human GLP1R was detectable in the lung of the homozygous B-hGLP1R mice, but not detectable in wildtype mice. and we proved that the protein could be expressed normally by western blot analysis. Human and mouse cross-reactive antibodies were used to detect the GLP1R of the wildtype and humanized GLP1R mice.
Protein expression analysis
IHC for human GLP1R expression. Pancreata from wild-type (+/+) and B-hGLP1R mice (H/H) mice were stained using an antibody specific for human GLP1R. and only the pancreas from B-hGLP1R mice (H/H) mice showed hGLP1R positive signal in plasma membrane.