Basic Information

Strain Name
C57BL/6J-Alcamtm1(ALCAM)Bcgen/Bcgen
Common Name
B-hALCAM mice
Background
C57BL/6J
Catalog Number
111146
Alias
ALCAM (MEMD; CD166)
NCBI Gene ID

Targeting Strategy

Gene targeting strategy for B-hALCAM mice.

Exons 2-14 of mouse Alcam, which encode the extracellular domain, were replaced by human ALCAM exons 2-14 in B-hALCAM mice.

ALCAM Expression in Blood Leukocytes

 

Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Blood from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H) was analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.

ALCAM Expression in Bone Marrow Populations

 

Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Bone marrow from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H) was analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.

ALCAM Expression in Splenocytes

 

Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Splenocytes from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H)  and analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.

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