Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hALCAM mice.
Exons 2-14 of mouse Alcam, which encode the extracellular domain, were replaced by human ALCAM exons 2-14 in B-hALCAM mice.
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ALCAM Expression in Blood Leukocytes
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Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Blood from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H) was analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.
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ALCAM Expression in Bone Marrow Populations
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Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Bone marrow from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H) was analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.
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ALCAM Expression in Splenocytes
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Strain specific ALCAM expression analysis in C57BL/6 wild-type mice and homozygous B-hALCAM mice by flow cytometry. Splenocytes from wild-type C57BL/6 mice (+/+) and homozygous B-hALCAM mice (H/H) and analyzed with species-specific anti-ALCAM antibody. Mouse ALCAM was exclusively detectable in wildtype mice. Human ALCAM was exclusively detectable in homozygous B-hALCAM mice.
