Basic Information

Strain name
C57BL/6-Fcgr4tm2(FCGR3A)/Bcgen
Common name
B-hCD16A mice
Background
C57BL/6
Catalog number
111173
Related gene
FCGR3A (Fc fragment of IgG receptor IIIa), CD16A
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD16A mice. The regulatory region and exons 1~5 of mouse Fcgr4 gene that encode the full-length protein were replaced by human FCGR3A exons 1~4 and regulatory region in B-hCD16A mice.

Protein expression analysis in NK cells

Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+)  and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was not detectable in NK cells of wild-type C57BL/6 mice and heterozygous B-hCD16A mice, which was consistent with the expected results. Human CD16A was exclusively detectable in NK cells of heterozygous B-hCD16A mice.

Protein expression analysis in granulocytes

Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+)  and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was detectable in granulocytes of wild-type C57BL/6 mice and heterozygous B-hCD16A mice. Human CD16A was not exclusively detectable in granulocytes of heterozygous B-hCD16A mice, which was consistent with the expected results.

Protein expression analysis in macrophages

Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type C57BL/6 mice(+/+)  and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was detectable both in PEMs of wild-type C57BL/6 mice and heterozygous B-hCD16A mice. Human CD16A was exclusively detectable in PEMs of heterozygous B-hCD16A mice.