Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD16A mice. The regulatory region and exons 1~5 of mouse Fcgr4 gene that encode the full-length protein were replaced by human FCGR3A exons 1~4 and regulatory region in B-hCD16A mice.
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Protein expression analysis in NK cells
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Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was not detectable in NK cells of wild-type C57BL/6 mice and heterozygous B-hCD16A mice, which was consistent with the expected results. Human CD16A was exclusively detectable in NK cells of heterozygous B-hCD16A mice.
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Protein expression analysis in granulocytes
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Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice(+/+) and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was detectable in granulocytes of wild-type C57BL/6 mice and heterozygous B-hCD16A mice. Human CD16A was not exclusively detectable in granulocytes of heterozygous B-hCD16A mice, which was consistent with the expected results.
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Protein expression analysis in macrophages
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Strain specific CD16A expression analysis in wild-type C57BL/6 mice and heterozygous B-hCD16A mice by flow cytometry. Peritoneal exudative macrophages(PEMs) were collected from wild-type C57BL/6 mice(+/+) and heterozygous B-hCD16A mice(H/+). Mouse Fcgr4 was detectable both in PEMs of wild-type C57BL/6 mice and heterozygous B-hCD16A mice. Human CD16A was exclusively detectable in PEMs of heterozygous B-hCD16A mice.
