Basic Information

Strain Name
C57BL/6-CD39tm1(hCD39)/Bcgen
Common Name
B-hCD39 mice
Background
C57BL/6
Catalog Number
110792
Related Gene
CD39 (ENTPD1, ectonucleoside triphosphate diphosphohydrolase 1)

Description

CD39, nucleoside triphosphate diphosphohydrolase-1 (NTPDase 1), is an ectoenzyme that degrades ATP to AMP. It is a member of ectonucleoside triphosphate dihydrolases (E-NTPDases) which are involved in regulation of extracellular nucleotide catabolism and controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is the dominant member of this family in the immune system and involved in suppression of inflammation and control of platelet activation. CD39 is expressed on B cells, dendritic cells, and a subset of T cells including regulatory T cells and memory T cells. The coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops. In human studies, it has been reported that CD4+CD25-CD39+ T cells are T inducers.

Details

Protein expression analysis

Strain specific CD39 expression analysis in heterozygous B-hCD39 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD39 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD39 antibody. Mouse CD39 was detectable in WT mice. Human CD39 was exclusively detectable in heterozygous B-hCD39 but not WT mice.

Strain specific CD39 expression analysis in heterozygous B-hCD39 mice by flow cytometry. Splenocytes were collected from WT and heterozygous B-hCD39 (H/+) mice, and analyzed by flow cytometry with species-specific anti-CD39 antibody. Mouse CD39 was detectable in WT mice. Human CD39 was exclusively detectable in heterozygous B-hCD39 but not WT mice.

Strain specific CD39 expression analysis in homozygous B-hCD39 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hCD39 (H/H) mice, and analyzed by flow cytometry with species-specific anti-CD39 antibody. Mouse CD39 was detectable in WT mice. Human CD39 was exclusively detectable in homozygous B-hCD39 but not WT mice.

Tumor growth curve

hCD39-MC38 tumor cells were subcutaneously implanted into the B-hCD39 mice. Tumor size and mice body weight were measured twice a week. Volume was expressed in mm3 using the formula: V=0.5a X b2 where a and b were the long and short diameters of the tumor, respectively(n=5). (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM. hCD39-MC38 tumor cells were able to establish tumor model steadily and can effectively track tumor volume.

In vivo efficacy of anti-human CD39 antibodies 

Antitumor activity of anti-human CD39 antibodies in B-hCD39 mice(v2). (A) Anti-human CD39 antibodies inhibit MC38 tumor growth in B-hCD39 mice. Murine colon cancer hCD39-MC38 cells were subcutaneously implanted into homozygous B-hCD39 mice(v2) (female, 6-7 week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with hCD39 antibodies with doses and schedules indicated in panel A. (B) Body weight changes during treatment. As shown in panel A, three hCD39 antibodies (in house) were efficacious in controlling tumor growth in B-hCD39 mice(v2), demonstrating they provide a powerful preclinical model for in vivo evaluation of anti-human CD39 antibodies. Values are expressed as mean ± SEM.

 

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