Basic Information

Strain Name
C57BL/6-Entpd1tm3(ENTPD1)/Bcgen
Common Name
B-hCD39 mice
Background
C57BL/6
Catalog Number
110783
Related Gene
CD39 (ENTPD1, ectonucleoside triphosphate diphosphohydrolase 1)
Targeting Strategy
The sequence that encodes the extracellular domain of human ENTPD1 was inserted following mouse Entpd1 exon 2 in B-hCD39 mice.

Description

CD39, nucleoside triphosphate diphosphohydrolase-1 (NTPDase 1), is an ectoenzyme that degrades ATP to AMP. It is a member of ectonucleoside triphosphate dihydrolases (E-NTPDases) which are involved in regulation of extracellular nucleotide catabolism and controlling the extracellular nucleoside triphosphate pool (NTP). CD39 is the dominant member of this family in the immune system and involved in suppression of inflammation and control of platelet activation. CD39 is expressed on B cells, dendritic cells, and a subset of T cells including regulatory T cells and memory T cells. The coordinated expression of CD39/CD73 on Tregs and the adenosine A2A receptor on activated T effector cells generates immunosuppressive loops. In human studies, it has been reported that CD4+CD25-CD39+ T cells are T inducers.

Details

Protein expression analysis

CD39 expression in B lymphocytes from wildtype and homozygous B-hCD39 mice. Splenocytes were collected from WT and homozygous B-hCD39 mice, and analyzed by flow cytometry with species-specific CD39 antibodies. Mouse CD39 was detectable in WT mice. Human CD39 was exclusively detectable in homozygous B-hCD39 mice but not WT mice.

CD39 expression analysis in T cells from homozygous B-hCD39 mice. Splenocytes were collected from WT and homozygous B-hCD39 mice, and analyzed by flow cytometry with species-specific CD39 antibody. Mouse CD39 was detectable in WT mice. Human CD39 was exclusively detectable in homozygous B-hCD39 mice but not WT mice.

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