Basic Information

Strain Name
C57BL/6-Cd47 tm1(CD47) /Bcgen
Stock Number
110010
Common Name
B-hCD47 mice
Source/Investigator
Bcgen (Beijing Biocytogen Co., Ltd)
Related Genes
Cd47 (CD47 antigen)
Species
C57BL/6
Appearance
Black
Genotypes
Homozygous

Description

CD47 is an integrin-associated protein (IAP) and is an immunoglobulin superfamily member. CD47 is widely expressed on cell surface and interacts with the signal regulatory protein α (SIRPα), thrombospondin-1 (TSP1) and integrins to mediate a series of responses including apoptosis, proliferation, and immunity. CD47 is an important “self” mark on the cell surface and inhibits macrophagocytosis by interacting with SIRPα on macrophage surface. Animal studies have shown that CD47 antibody is an effective treatment for multiple types of tumors. Therefore, CD47 is a promising new target for tumor immunotherapy following PD- 1/PD-L1 axis.

Targeting Strategy

Details

Phenotype

mRNA Expression Analysis

B-hCD47-Mice-details-mrna-expression-analysis

RT-PCR analysis of CD47 mRNA expression. The CD47, but not mCD47, mRNA was detectable in splenocytes of the homozygous B-hCD47 mice.

Protein Expression Analysis

Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hCD47 mice were analyzed by flow cytometry. Splenocytes from WT mice express murine CD47 while human CD47 was detectable on spleen cells from B-hCD47 homozygous mice.

 

Application

Human CD47 mAb toxicity evaluation

 

Homozygous B-hCD47 mice were injected with various anti-hCD47 antibodies via i.p. injection. Body weight was measured daily. The results indicated toxicity of different antibodies. hCD47 Ab4 and hCD47 Ab6 showed high toxicity levels, as mice died. Y-axis showed average body weight ± SEM.

Human CD47 mAb toxicity evaluation

B-hCD47-Mice-details-Human-CD47-mAb-toxicity-evaluation2

 

Homozygous B-hCD47 mice were i.p. injected with anti-hCD47 antibodies. Blood from each mouse were collected and Blood Routine Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.

 

B-hCD47-Mice-details-Human-CD47-mAb-toxicity-evaluation3

Homozygous B-hCD47 mice were I.P. injected with anti-hCD47 antibodies. Blood from each mouse were collected and Blood Biochemical Test was conducted. Blood from Ab3 and Ab5 groups were not analyzed because of death of the treated mice.

References

1. PNAS July 2, 2013 110 (27) 11103-11108; doi: 10.1073/pnas.1305569110
2. Front. Immunol., 21 April 2017 doi: 10.3389/fimmu.2017.00404
3. J. Thorac Dis 2017;9(2): E168-E174 doi: 10.21037/jtd.2017.02.30

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