Basic Information
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Targeting strategy
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Gene targeting strategy for B-hCD19 mice.
The coding sequence of human extracellular domain, mouse transmembrane and cytoplasmic domain was inserted into murine exon 2 in B-hCD19 mice.
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Protein expression analysis of CD48 in T cells, CD4+T cells and CD8+T cells of splenocytes
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Strain specific CD48 expression analysis in homozygous B-hCD48 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD48 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD48 antibody. Mouse CD48 was detectable in T cells (A), CD4+T cells (B) and CD8+T cells (C) of wild-type C57BL/6 mice. Human CD48 was exclusively detectable in T cells (A), CD4+T cells (B) and CD8+T cells (C) of homozygous B-hCD48 mice but not in wild-type C57BL/6 mice.
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Protein expression analysis of CD48 in NK cells, B cells and dendritic cells of splenocytes
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Strain specific CD48 expression analysis in homozygous B-hCD48 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD48 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD48 antibody. Mouse CD48 was detectable in NK cells (A), B cells (B) and dendritic cells (C) of wild-type C57BL/6 mice. Human CD48 was exclusively detectable in NK cells (A), B cells (B) and dendritic cells (C) of homozygous B-hCD48 mice but not in wild-type C57BL/6 mice.
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Protein expression analysis of CD48 in monocytes, macrophages and granulocytes of splenocytes
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Strain specific CD48 expression analysis in homozygous B-hCD48 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD48 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD48 antibody. Mouse CD48 was detectable in monocytes (A), macrophages (B) and granulocytes (C) of wild-type C57BL/6 mice. Human CD48 was exclusively detectable in monocytes (A), macrophages (B) and granulocytes (C) of homozygous B-hCD48 mice but not in wild-type C57BL/6 mice. -
Protein expression analysis of CD48 in T cells, CD4+T cells and CD8+T cells of lymph nodes
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Strain specific CD48 expression analysis in homozygous B-hCD48 mice by flow cytometry. Lymph nodes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD48 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD48 antibody. Mouse CD48 was detectable in T cells (A), CD4+T cells (B) and CD8+T cells (C) of wild-type C57BL/6 mice. Human CD48 was exclusively detectable in T cells (A), CD4+T cells (B) and CD8+T cells (C) of homozygous B-hCD48 mice but not in wild-type C57BL/6 mice.
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Protein expression analysis of CD48 in NK cells and B cells of lymph nodes
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Strain specific CD48 expression analysis in homozygous B-hCD48 mice by flow cytometry. Lymph nodes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD48 mice (H/H), and analyzed by flow cytometry with species-specific anti-CD48 antibody. Mouse CD48 was detectable in NK cells (A) and B cells (B) of wild-type C57BL/6 mice. Human CD48 was exclusively detectable in NK cells (A) and B cells (B) of homozygous B-hCD48 mice but not in wild-type C57BL/6 mice.
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Summary
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Protein expression analysis:
Human CD48 was both detectable in spleen and lymph nodes of homozygous B-hCD48 mice, and mouse Cd48 was detectable only in wild-type C57BL/6 mice.
