Basic Information

Strain Name
C57BL/6-Garptm1(GARP)TGFB1tm1(TGFB1)/Bcgen
Common Name
B-hGARP/hTGFB1 mice
Background
C57BL/6N
Catalog number
112241
Related Genes
GARP(LRRC32,CPPRDD,D11S833E);  TGFB1(CED,LAP,DPD1,TGFB,IBDIMDE,TGFbeta,TGF-beta1)
NCBI Gene ID
GARP: 2615; TGFB1: 7040

Gene targeting strategy

Gene targeting strategy for B-hGARP/TGFB1 mice. The exons 1~2 of mouse Garp gene that encode the extracellular domain were replaced by human GARP exon 1~2 in B-hGARP/TGFB1 mice. The human TGFB1 gene that encodes the full-length protein was inserted following part of the exon 2 of the mouse Tgfb1 gene in the B-hGARP/hTGFB1 mice.

Protein expression analysis

Species-specific GARP protein expression analysis in wild-type and humanized B-hGARP/hTGFB1 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hGARP/hTGFB1 (H/H) mice and analyzed by flow cytometry using species-specific anti-GARP antibodies. Murine GARP protein was detected in wild-type mice, while human GARP protein was detected in B-hGARP/hTGFB1 mice.

Species-specific TGFB1 protein expression analysis in wild-type and humanized B-hGARP/hTGFB1 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hGARP/hTGFB1 (H/H) mice and analyzed by flow cytometry using species-specific anti-TGFB1 antibodies. Murine TGFB1 protein was detected in wild-type mice, while human TGFB1 protein was detected in B-hGARP/hTGFB1 mice.

Species-specific GARP protein expression analysis in wild-type and humanized B-hGARP/hTGFB1 mice. Blood was extracted from wild-type C57BL/6 (+/+) and homozygous B-hGARP/hTGFB1 (H/H) mice and analyzed by flow cytometry using species-specific anti-GARP antibodies. Murine GARP protein was detected in wild-type mice, while human GARP protein was detected in B-hGARP/hTGFB1 mice.

Species-specific TGFB1 protein expression analysis in wild-type and humanized B-hGARP/hTGFB1 mice. Blood was extracted from wild-type C57BL/6 (+/+) and homozygous B-hGARP/hTGFB1 (H/H) mice and analyzed by flow cytometry using species-specific anti-TGFB1 antibodies. Murine TGFB1 protein was detected in wild-type mice, while human TGFB1 protein was detected in B-hGARP/hTGFB1 mice.

Antibody binding assay

Antibody binding assay using wild-type and humanized B-hGARP/hTGFB1 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hGARP/hTGFB1 (H/H) mice and analyzed by flow cytometry to assess anti-human GARP/latent-TGFB1 antibody ABBV151 (10ug/mL) binding. Tregs from homozygous B-hGARP/hTGFB1 mice showed enhanced binding potential to ABBV151 versus isotype control.