Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hNKP46 mice(CB-17 SCID). The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice(CB-17 SCID).
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Protein expression analysis
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Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(CB-17 SCID). Splenocytes were isolated from wild-type CB-17 SCID (+/+) and heterozygous B-hNKP46 mice(CB-17 SCID) (H/+) and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous B-hNKP46 mice(CB-17 SCID) mice, while human NKP46 protein was only detected in B-hNKP46 mice(CB-17 SCID).
Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(CB-17 SCID). Splenocytes were isolated from wild-type CB-17 SCID (+/+) and heterozygous B-hNKP46 mice(CB-17 SCID) (H/+) and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous B-hNKP46 mice(CB-17 SCID) mice, while human NKP46 protein was only detected in B-hNKP46 mice(CB-17 SCID).
