Basic Information

Strain Name
CB17/lcr-PrkdcscidNcr1tm1(NCR1)/Bcgen
Common Name
B-hNKP46 mice(CB-17 SCID)
Background
CB-17 SCID
Catalog number
112259
Aliases
CD335, LY94, NK-p46, NKP46
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hNKP46 mice(CB-17 SCID). The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice(CB-17 SCID).

Protein expression analysis

 

Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(CB-17 SCID). Splenocytes were isolated from wild-type CB-17 SCID (+/+) and heterozygous B-hNKP46 mice(CB-17 SCID) (H/+) and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous B-hNKP46 mice(CB-17 SCID) mice, while human NKP46 protein was only detected in B-hNKP46 mice(CB-17 SCID).

Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(CB-17 SCID). Splenocytes were isolated from wild-type CB-17 SCID (+/+) and heterozygous B-hNKP46 mice(CB-17 SCID) (H/+) and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous B-hNKP46 mice(CB-17 SCID) mice, while human NKP46 protein was only detected in B-hNKP46 mice(CB-17 SCID).