Basic Information
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mRNA Expression Analysis
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Species–specific OX40L mRNA expression analysis in double humanized B-hOX40/hOX40L mice. Dendritic cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hOX40/hOX40L (H/H) mice and analyzed by RT-PCR for OX40L expression. Human OX40L mRNA was exclusively detected in B-hOX40/hOX40L mice compared to wild-type mice.
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Protein Expression Analysis
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Species–specific OX40 expression analysis in double humanized B-hOX40/hOX40L mice. Following anti-CD3ε stimulation in vivo, splenocytes were collected from wild-type C57BL/6 (+/+) and homozygous B-hOX40/hOX40L (H/H) mice and analyzed by flow cytometry using species-specific anti-OX40 antibodies. Human OX40 was exclusively detected in B-hOX40/hOX40L mice compared to wild-type mice.
Species–specific OX40L expression analysis in double humanized B-hOX40/hOX40L mice. Bone marrow cells or bone marrow-derived DCs stimulated with LPS were collected from wild-type C57BL/6 (+/+) and homozygous B-hOX40/hOX40L (H/H) mice and analyzed by flow cytometry using species-specific anti-OX40L antibodies. Human OX40L was exclusively detected in B-hOX40/hOX40L mice compared to wild-type mice.
