Basic Information

Strain name
C57BL/6-Pvrigtm1(PVRIG)/Bcgen
Common name
B-hPVRIG mice
Background
C57BL/6
Catalog number
110091
Aliases
CD112R; C7orf15
NCBI Gene ID

mRNA expression analysis

Strain specific analysis of PVRIG gene expression in WT and B-hPVRIG mice by RT-PCR. Mouse Pvrig mRNA was detectable in splenocytes of wild-type (+/+). Human PVRIG mRNA was detectable only in B-hPVRIG mice (H/H) but not in wild type mice.

Protein expression analysis

PVRIG expression analysis in homozygous B-hPVRIG mice by western blot. Heart collected from wild type and homozygous B-hPVRIG mice (H/H) and analyzed by western blot with anti-PVRIG antibody. Mouse PVRIG was detectable in wild-type mice. Human PVRIG was detectable in homozygous B-hPVRIG mice. Anti-PVRIG antibody is crossly reactive with PVRIG in human and mice.

In vivo efficacy of anti-human PVRIG antibodies

Antitumor activity of anti-human PVRIG antibodies in B-hPVRIG mice. (A) COM-701 (in house) and SRF-813 (in house) inhibited MC38 tumor growth in B-hPVRIG mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPVRIG mice (female, 6-week-old, n=5). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with COM-701 (in house) and SRF-813 (in house) with doses and schedules in panel A. (B) Body weight changes during treatment. As shown in panel A, anti-human PVRIG antibodies were efficacious in controlling tumor growth in B-hPVRIG mice, demonstrating that the B-hPVRIG mice provide a powerful preclinical model for in vivo evaluation of anti-human PVRIG antibodies. Values are expressed as mean ± SEM.

Summary

mRNA expression analysis:

Mouse Pvrig mRNA was detectable in splenocytes of wild-type (+/+). Human PVRIG mRNA was detectable in B-hPVRIG mice (H/H) but not in wild-type mice.

Protein expression analysis:

Mouse PVRIG was detectable in wild-type mice. Human PVRIG was detectable in homozygous B-hPVRIG mice.

In vivo efficacy

Anti-human PVRIG antibodies were efficacious in controlling tumor growth in B-hPVRIG mice.