Basic Information

Strain name
C57BL/6-Pvrigtm1(PVRIG)/Bcgen
Common name
B-hPVRIG mice
Background
C57BL/6J
Catalog number
110091
Aliases
CD112R; C7orf15
NCBI Gene ID

mRNA expression analysis

Species-specific PVRIG gene expression analysis in wild-type and humanized B-hPVRIG mice by RT-PCR. Murine Pvrig mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human PVRIG mRNA was exclusively detected in homozygous B-hPVRIG (H/H) mice.

Protein expression analysis

Species-specific PVRIG protein expression analysis in wild-type and humanized B-hPVRIG mice. Cardiac tissue was isolated from wild-type C57BL/6 (+/+) and homozygous B-hPVRIG (H/H) mice and analyzed by western blot using an anti-m/hPVRIG antibody. Murine and human PVRIG protein was detected in wild-type and B-hPVRIG mice.

In vivo Efficacy of Anti-Human PVRIG Antibodies

Antitumor activity of anti-human PVRIG antibodies in B-hPVRIG mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hPVRIG (H/H) mice (female, 6-week-old, n=5). Mice were grouped when tumor volume reached ~100 mm3, at which time they were treated with anti-human PVRIG antibodies (COM-701, SRF-813, in house). (A) COM-701 and SRF-813 antibodies controlled tumor growth in B-hPVRIG mice, (B) without negatively impacting body weight changes. Values are expressed as mean ± SEM.

Poster

AACR 2022: Humanized PVRIG Mice: A Novel Tool for Evaluating Anti-PVRIG Immunotherapies