Protein Expression Analysis
Splenocytes from both wild type (WT) C57BL/6 and homozygous B-hSIRPα /hCD47 mice were analyzed by flow cytometry. Mouse CD47+ T cells were detectable in the C57BL/6 mice, mouse SIRPα + T cells were detectable in both WT C57BL/6 mice and homozygous B-hSIRPα / hCD47 mice, while human CD47+ and SIRPα + T cells were detectable in the homozygous B-hSIRPA/hCD47 mice. This might result from the cross-recognition of hSIRPα by anti-mSIRPα antibodies
Human CD47 mAb Efficacy and Toxicity Evaluation (MC38-hCD47 Cell Line)
Colon cancer MC38-hCD47 cells were subcutaniously implanted into thehomozygous hSIRPα /hCD47 mice. The mice were divided into control andtreatment groups as the tumor volume was 150±50 mm3 (n=5). The resultindicated that B-hSIRPα /hCD47 mice model was an effective tool for in vivo hCD47 antibody efficacy study. The average ±SEM of tumor sizes are shown in the figure.
Human CD47 mAb Toxicity Evaluation
Anti-hCD47 antibodies were i.p. injected into homozygous hSIRPα/hCD47 mice. Blood were collected two days after the treatment and analyzed by Blood Routine Test.
Anti-hCD47 antibodies were i.p. injected into homozygous hSIRPα/hCD47 mice.Blood were collected two days after the treatment and analyzed by Blood Biochemical Test.
Anti-hCD47 antibodies were i.p. injected into homozygous hSIRPα/hCD47 mice. Blood was collected two days after the treatment and analyzed by complete blood count.
Blood were collected at the endpoint of the treatment and analyzed by Blood Biochemical Test.
Human SIRPα mAb Efficacy Evaluation (MC38-hCD47 Cell Line)
Murine colon cancer MC38-hCD47 cells were subcutaneously implanted into homozygous B-hSIRPα/hCD47 mice. Mice were grouped when the tumor size was approximately 150±50 mm3 (n=6). Three human SIRPα antibodies differently inhibited tumor growth, confirming that the B-hSIRPα/hCD47 mouse model is a powerful tool for in vivo SIRPα antibody pharmacological efficacy study. (A) Tumor average volume ± SEM, (B) Mice average weight ± SEM.
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