Gene targeting strategy for B-hVSIG4 mice. Coding sequences of human VSIG4 and mouse 3’UTR were inserted into exon 2 of mouse Vsig4 gene. Mouse Vsig4 gene was replaced with human VSIG4 gene.
mRNA expression analysis
Strain specific analysis of VSIG4 gene expression in wild-type C57BL/6 mice and homozygous B-hVSIG4 mice by RT-PCR. Mouse Vsig4 mRNA was detectable in liver tissue of wild-type C57BL/6 mice (+/+). Human VSIG4 mRNA was detectable only in homozygous B-hVSIG4 mice but not in wild-type mice.
In vivo efficacy of anti-human VSIG4 antibodies
Anti-tumor activity of anti-human VSIG4 antibody in humanized B-hVSIG4 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hVSIG4 mice (female, 7-week-old, n=5). (A) Anti-human VSIG4 antibody (in house analog) inhibited MC38 tumor growth in B-hVSIG4 mice, (B) without negatively impacting body weight.
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