human HSC (hematopoietic stem cells ) engrafted humanized mice are a powerful model allows researchers to examine the human immune system and related primates‘ alien virus research and cellular immunotherapy preclinical evaluation. However, maintenance, differentiation, and function of human hematopoietic cells are suboptimal in these hosts. Thrombopoietin (THPO) has been demonstrated as a crucial cytokine supporting maintenance and self-renewal of HSCs. Moreover, a species barrier between human immune cells and the mouse microenvironment limits human acquired as well as innate immune cell development. Such as frequencies of human macrophages cells did not reach physiological levels in B-NDG humanized mice. Cytokines CSF1 has been reported controls the production, differentiation, and function of monocytes and macrophages cells. Biocytogen developed the B-NDG hCSF1/hTHPO mice in which we replaced the gene encoding mouse Csf1/Thpo by its human CDS region. This mouse combines a B-NDG mouse background and expresses human hCSF1/hTHPO protein, will become a suitable animal model to investigate development and function of human macrophage cells without irradiation treatment.
HSC Human Immune System Engraftment
Human CD34+ cells (0.15 M) were intravenously injected into homozygote B-NDG hCSF1/hTHPO mice (female, 6 week-old, n=15). Representative flow cytometric analysis of peripheral blood lymphocyte from mice after engraftment with human CD34+ cells. human multi-lineage cells, including T, B, NK, myeloid cells，monocyte and neutrophils, were successfully constructed. Our results suggest that the human HSC (hematopoietic stem cells) engrafted humanized mice model was successfully constructed.