Basic Information
Description
Whereas humanized mouse models have contributed significantly to human immunology research, human T cells developing in mouse thymic environment fail to demonstrate HLA-restricted function.To achieve HLA-restricted human immune response, we created NOD/SCID/IL2rg null strain (B-NDG) with homozygous expression of HLA class I heavy chain and lightchain (B-NDG HLA 2.1 mice). The model B2m gene (Exon1 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS and HLA-A*0201 gene. The HLA-expressing humanized mouse with functional HLA-restricted T cells and consistent representation of rare T-cell subsets overcomes a major constraint in human immunology, and serves as a useful model for investigation of human immune responses against pathogens and for the development of therapeutic strategies against human diseases.
-
Details
-
Protein expression analysis (heterozygous mice)
Strain specific B2M and HLA expression analysis in heterozygous B-NDG HLA-A2.1 mice by flow cytometry. Splenocytes from both wild type (+/+) C57BL/6 and heterozygous B-NDG HLA-A2.1 (H/+) mice were analyzed by flow cytometry. Mouse B2M and H-2K/H-2D were detectable in the WT C57BL/6 and heterozygous B-NDG HLA-A2.1 mice. Human B2M and HLA-A2 were only detectable in the heterozygous B-NDG HLA-A2.1 mice.
