B-NDG MHC I/II DKO mice plus

Basic Information

Strain Name
NOD.CB17-PrkdcscidIL2rgtm1B2mtm1Fcgrttm1(B2m/Fcgrt)H2-Ab1tm1/Bcgen
Common Name
B-NDG B2m plus/H2-Ab1 KO mice
Background
B-NDG
Catalog number
111895
Related Genes
H2-Ab1: AI845868, Abeta, H-2Ab, H2-Ab, I-Abeta, IAb, Ia-2, Ia2, Rmcs1; B2m: Ly-m11, beta2-m, beta2m; Fcgrt: FcRn
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-NDG MHC I/II DKO mice plus. The murine B2m and H2-Ab1 gene were knocked out while a fused gene composed of murine B2m and Fcgrt gene was inserted after the signal peptide sequence of murine Fcgrt gene in B-NDG MHC I/II DKO mice plus.

Protein expression analysis

Strain specific H-2Kb/H-2Db (MHC-I) and I-Ak (MHC-II) expression analysis in B-NDG mice, B-NDG B2m KO plus mice and B-NDG B2m plus/H2-Ab1 KO mice by flow cytometry.  Splenocytes were collected from the three mice and analyzed by flow cytometry. Mouse H-2Kb/H-2Db was only detectable in B-NDG mice but not in B-NDG B2m KO plus mice and BNDG B2m plus/H2-Ab1 KO mice. Mouse I-Ak was only detectable in B-NDG mice and B-NDG B2m KO plus mice but not in B-NDG B2m plus/H2-Ab1 KO mice.