Basic Information

Common Name
B-hCD38 mice
Background
C57BL/6N
Catalog Number
110046
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hCD38 mice. The CDS of human CD38 encoding the extracellular domain was inserted following partial exon 2 of  mouse Cd38 in B-hCD38 mice.

mRNA expression analysis

Species-specific CD38 gene expression analysis in wild-type and humanized B-hCD38 mice by RT-PCR. Murine Cd38 mRNA was detected in splenocytes isolated from wild-type (+/+) mice, while human CD38 mRNA was exclusively detected in B-hCD38 mice.

Protein expression analysis

Species-specific CD38 protein expression analysis in humanized B-hCD38 mice. Splenocytes and blood cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD38 (H/H) mice and analyzed by flow cytometry using species-specific anti-CD38 antibodies. Murine CD38 protein was detected in wild-type mice, while human CD38 protein was exclusively detected in B-hCD38 mice.

Complete blood count & blood chemistry results

Complete blood count (CBC) in wild-type and humanized B-hCD38 mice. Blood from wild-type C57BL/6 and humanized B-hCD38 mice (n=5, 6 week-old, female and male) was isolated and analyzed for CBC. Levels between B-hCD38 and wild-type mice were similar, indicating that CD38 humanization does not change blood cell composition and morphology. Values are expressed as mean ± SEM.

 

Blood chemistry tests in wild-type and humanized B-hCD38 mice. Serum was collected from wild-type C57BL/6 and B-hCD38 mice (n=5, 6 week-old, female and male) and analyzed for levels of ALT, AST and other indicators. Levels between B-hCD38 and wild-type mice were similar, indicating that CD38 humanization does not change organ health. Values are expressed as mean ± SEM.

Anti-human CD38 antibody binding potential

Analysis of splenocytes in humanized B-hCD38 mice. Splenocytes were isolated from wild-type C57BL/6 and homozygous B-hCD38 mice (female, 6-week-old) and analyzed by flow cytometry to assess anti-human CD38 antibody binding. Single live cells were gated on CD19+ and used for further analysis as indicated. Splenocytes from B-hCD38 mice displayed higher binding potential to the anti-hCD38 antibody (daratumumab, in house) compared to isotype control.