Basic Information
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Targeting strategy
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Gene targeting strategy for B-HLA-A11.1 mice. The B2M gene (Exon1 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS and HLA-A*1101 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H-2Db gene containing the α3, transmembrane and cytoplasmic domains.
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Protein expression analysis
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Strain specific B2M and HLA-A expression analysis in homozygous B-HLA-A11.1 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-HLA-A11.1 mice (H/H) and analyzed by flow cytometry with species-specific anti-B2M and anti-HLA-A antibodies. Mouse B2M and H-2Db were detectable in wild-type C57BL/6 mice. Human B2M and HLA-A11.1 were exclusively detectable in homozygous B-HLA-A11.1 mice but not in wild-type mice.
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Analysis of leukocytes cell subpopulation in spleen
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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and B-HLA-A11.1 mice (n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of total T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-HLA-A11.1 mice were similar to those in the C57BL/6 mice. Percent of CD8+ T cells were significantly decreased, demonstrating that introduction of hB2M-HLA-A11.1-H-2D in place of mouse B2M may affected the development of CD8 + T cells, which in turn affected the proportion of T cell subtypes in the spleen. Values are expressed as mean ± SEM.
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Analysis of leukocytes cell subpopulation in blood
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Analysis of blood leukocyte subpopulations by FACS. Blood cells were isolated from female C57BL/6 and B-HLA-A11.1 mice (n=3, 8-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of total T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-HLA-A11.1 mice were similar to those in the C57BL/6 mice. Percent of CD8+ T cells were significantly decreased, demonstrating that introduction of hB2M-HLA-A11.1-H-2D in place of mouse B2M may affected the development of CD8 + T cells, which in turn affected the proportion of T cell subtypes in the blood. Values are expressed as mean ± SEM.
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Summary
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- Protein expression analysis:
Mouse B2M and H-2Db were detectable in wild-type C57BL/6 mice. Human B2M and HLA-A11.1 were exclusively detectable in homozygous B-HLA-A11.1 mice, while mouse B2M and/ H-2Db were not detectable in the homozygous B-HLA-A11.1 mice.
- Leukocytes cell subpopulation analysis：
Introduction of hB2M-HLA-A11.1-H-2D in place of mouse B2M may affected the development of CD8+ T cells, which in turn affected the proportion of T cell subtypes in spleen, lymph nodes and blood.
