PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
NCBI Gene ID
Gene targeting strategy
Gene targeting strategy for B-hPSMA mice. The extracellular coding sequence of human PSMA gene and mouse 3’ UTR were inserted into mouse Psma exon 2 in B-hPSMA mice, so the mouse Psma gene was disrupted. The intracellular and transmembrane sequence of mouse Psma did not change.
Protein expression analysis
IHC for human PSMA expression. Kidney tissues from wild-type (+/+) and B-hPSMA mice (H/H) were stained with an antibody specific for human PSMA, and only the kidney tubular epithelial cells from B-hPSMA mice (H/H) showed hPSMA expression. (C57BL/6-1: kidney tissues from C57BL/6 mice were only stained with anti-IgG antibody; Human,C57BL/6-2 and B-hPSMA mice kidney tissues were stained with anti-human PSMA antibody).