Basic Information
Strain name
C57BL/6-Folh1tm1(FOLH1)/Bcgen
Common name
B-hPSMA mice
Catalog number
110119
Background
C57BL/6N
Aliases
PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
NCBI Gene ID
-
Gene targeting strategy
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Gene targeting strategy for B-hPSMA mice. The extracellular coding sequence of human PSMA gene and mouse 3’ UTR were inserted into mouse Psma exon 2 in B-hPSMA mice, so the mouse Psma gene was disrupted. The intracellular and transmembrane sequence of mouse Psma did not change.
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Protein expression analysis
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IHC for human PSMA expression. Kidney tissues from wild-type (+/+) and B-hPSMA mice (H/H) were stained with an antibody specific for human PSMA, and only the kidney tubular epithelial cells from B-hPSMA mice (H/H) showed hPSMA expression. (C57BL/6-1: kidney tissues from C57BL/6 mice were only stained with anti-IgG antibody; Human,C57BL/6-2 and B-hPSMA mice kidney tissues were stained with anti-human PSMA antibody.)
