Basic Information

Strain name
C57BL/6-Folh1tm1(FOLH1)/Bcgen
Common name
B-hPSMA mice
Catalog number
110119
Background
C57BL/6N
Aliases
PSM; FGCP; FOLH; GCP2; PSMA; mGCP; GCPII; NAALAD1; NAALAdase
NCBI Gene ID

Gene targeting strategy

Gene targeting strategy for B-hPSMA mice. The extracellular coding sequence of human PSMA gene and mouse 3’ UTR were inserted into mouse Psma exon 2 in B-hPSMA mice, so the mouse Psma gene was disrupted. The intracellular and transmembrane sequence of mouse Psma did not change.

Protein expression analysis

IHC for human PSMA expression. Kidney tissues from wild-type (+/+) and B-hPSMA mice (H/H) were stained with an antibody specific for human PSMA, and only the kidney tubular epithelial cells from B-hPSMA mice (H/H) showed hPSMA expression. (C57BL/6-1: kidney tissues from C57BL/6 mice were only stained with anti-IgG antibody; Human,C57BL/6-2 and B-hPSMA mice kidney tissues were stained with anti-human PSMA antibody).

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