• 310825
| Product name | B-hFAP MC38 |
|---|---|
| Catalog number | 310825 |
| Strain background | C57BL/6 |
| Aliases | DPPIVA, FAPalpha, SIMP |
| Tissue | Colon |
| Disease | Colon carcinoma |
| Species | Mouse |
| Application | B-hFAP MC38 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe exogenous promoter and the CDS of human FAP was inserted to replace murine exons 7-9 in B-hFAP MC38 cells. Human FAP is highly expressed on the surface of B-hFAP MC38 cells.
Tageting strategyGene targeting strategy for B-hFAP MC38 cells. The exogenous promoter and the CDS of human FAP were inserted to replace murine exons 7-9. The insertion disrupts the endogenous murine Fap gene, resulting in a non-functional transcript.
Protein expression analysis
FAP expression analysis in B-hFAP MC38 cells by flow cytometry. Single cell suspensions from B-hFAP MC38 cultures were stained with species-specific anti-FAP antibody. Human FAP was detected on the surface of B-hFAP MC38 but as the generation increases, the expression decreases.
Protein expression analysis of tumor cells
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells (1x106) and wild-type MC38 cells (1x106) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
Tumor growth curve of individual mice
B-hFAP MC38 tumor growth of individual mice. B-hFAP MC38 cells (1x106) and wild-type MC38 cells (1x106) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). As shown in panel, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve & Body weight changes
Subcutaneous homograft homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
Tumor growth curve of individual mice
B-hFAP MC38 tumor growth of individual mice. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-hCD3E mice (female, 9-week-old, n=6). As shown in panel, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hFAP MC38 cells. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). Tumor volume and body weight were measured twice a week. (A) Average tumor volume. (B) Body weight. Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies. Values are expressed as mean ± SEM.
Tumor growth curve of individual mice
B-hFAP MC38 tumor growth of individual mice. B-hFAP MC38 cells and wild-type MC38 cells were subcutaneously implanted into B-h4-1BB mice (female, 6-week-old, n=6). As shown in panel, B-hFAP MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.
