Origin: The MC38 cell line is derived from C57BL/6 murine colon adenocarcinoma cells. The cell line is a commonly used murine model for colorectal carcinoma.
Background Information: HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen so that they can be recognized by cytotoxic T cells. MAGEA2 is a member of the MAGEA gene family. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita.
Gene targeting strategy: The B2m gene (Exon2 to Exon3) of mouse were replaced by the sequence encompassing the human B2M CDS, HLA-A*0201 gene that included leader sequence, α1 and α2 domains ligated to a fragment of the murine H-2Db gene containing the α3, transmembrane and cytoplasmic domains and human MAGEA2 CDS. Human HLA-A2.1 is highly expressed on the surface of B-HLA-A2.1/hMAGEA2 MC38. Human MAGEA2 is detectable by western blot.
Tumorigenicity: Confirmed in B-HLA-A2.1 mice.
Application: The B-HLA-A2.1/hMAGEA2 MC38 tumor model can be used for preclinical evaluation of cancer vaccines and TCR mimics.
Notes:
- Inoculated cell lines can be suspended with RPMI-1640 stock solution.
- Before implementing the project, it is recommended to perform tumor growth experiments. The recommended cell inoculation amount is between 5E5-1E6.
- In the experiment, it is necessary to ensure that the number of animals inoculated subcutaneously is at least 1.6 times the actual grouping number.
