Description
MUC17: MUC17 is a membrane-associated mucin involved in epithelial protection, cell survival, and tumor progression.
- Gene Information: MUC17 (Mucin 17) is a membrane-bound glycoprotein encoded on human chromosome 7 and belongs to the mucin family involved in epithelial barrier function.
- Protein Expression: MUC17 is primarily expressed on gastrointestinal epithelial cells, especially in the small intestine and colon, and is aberrantly expressed in several gastrointestinal cancers.
- Signaling Pathway: MUC17 regulates epithelial homeostasis through pathways associated with cell proliferation, survival, and migration, including EGFR-related signaling.
- Therapeutic Targeting: Targeting MUC17 with antibodies, ADCs, and other biologics may enable selective elimination of MUC17-expressing tumor cells, representing a promising strategy for gastrointestinal cancers.
Targeting strategy
MUC17
- The exons 5-10 of mouse Muc17 gene that encode the human SEA domain and part of the extracellular domain were replaced by human MUC17 exons 5-10 in B-hMUC17 mice.
mRNA Expression by RT-PCR
- Mouse Muc17 mRNA was detectable only in small intestines of wild-type C57BL/6 mice.
- Human MUC17 mRNA was detectable only in homozygous B-hMUC17 mice, but not in wild-type mice.
mRNA MUC17 were detectable in B-hMUC17 mice by RT-PCR and sequencing. Small intestines were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hMUC17 mice (H/H). Primers were designed to detect the human and mouse MUC17. Human MUC17 mRNA was detectable in B-hMUC17 mice, but not in wild-type mice. Sequencing of the PCR products confirmed that the amplified sequences were consistent with database reference sequences.
MUC17 Protein Expression
- Human MUC17 was exclusively detectable in homozygous B-hMUC17 mice, but not in wild type mice.
Strain specific MUC17 expression analysis in homozygous B-hMUC17 mice by flow cytometry. Single-cell suspension was obtained from intestinal tissue homogenates of wild type mice (+/+) and homozygous B-hMUC17 mice (H/H), and analyzed by flow cytometry with anti-human MUC17 antibody (Biolegend, 395306).
* When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hMUC17 mice] (Cat# 111292) was purchased from Biocytogen.
