Orthotopic Colorectal Cancer Model

Orthotopic Colorectal Cancer Model

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    Results
    Orthotopic Colorectal Cancer Model: B-Tg(Luc) HCT 116 in B-NDG Mice
    Orthotopic Colorectal Cancer Model: B-Tg(Luc) HCT 116 in B-NDG Mice
    Establishment of orthotopic colorectal cancer model.
    The B-NDG mice were inoculated with a volume of 40 μL of B-Tg(Luc) HCT 116 cell suspension (1×105, 5×105, 1×106), and the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased and body weight of mice had modest weight loss over time. This indicated that the cell line was successfully constructed as an orthotopic tumor model.
    Orthotopic Colorectal Cancer Model: B-luc CT26.WT in Balb/c Mice
    Orthotopic Colorectal Cancer Model: B-luc CT26.WT in Balb/c Mice
    Establishment of orthotopic colorectal cancer model.
    The B-NDG mice (F, n=6) were inoculated with B-Luc CT26.WT cell suspension. Subcutaneous tumor formation of B-Luc CT26.WT is slower than that of wild-type cells. and the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased and body weight of mice had modest weight loss over time. This indicated that the cell line was successfully constructed as an orthotopic tumor model.
    Orthotopic Colorectal Cancer Model: B-luc-GFP HT-29 in B-NDG Mice
    Orthotopic Colorectal Cancer Model: B-luc-GFP HT-29 in B-NDG Mice
    Establishment of orthotopic colorectal cancer model.
    The colon of B-NDG mice were inoculated with B-luc-GFP HT-29 cell suspension, and the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased and body weight of mice had modest weight loss over time. This indicates that the cell line was successfully constructed as an orthotopic tumor model. This indicates that the cell line was successfully constructed as an orthotopic tumor model.
    Study ID: Groups Animal number Treatment Dosage (mg/kg) Lot No. Dosing Volume Dosing Route Treatment Schedule Treatment Times
    24P071529 G1 6 PBS - 2933643 10μL/g i.v. QW 2
    G2 6 DS-8201 3 394042 10μL/g i.v. QW 2
    G3 6 DS-8201 6 394042 10μL/g i.v. QW 2
    B-luc-GFP HT-29 in B-NDG Mice img 2
    Antitumor activity of anti-HER2 antibody in B-luc-GFP HT-29 colorectal orthotopic model.The DS-8201 significantly inhibited B-luc-GFP HT-29 tumor growth in B-NDG mice. B-luc-GFP HT-29 cells (5×10⁴) suspension mixed in Matrigel solution (1:1) was orthotopically implanted into B-NDG mice (female, 7 week-old, n=6). Mice were grouped when Imaging signal value reached approximately 2×10⁸ p/sec, at which time they were treated with the DS-8201 with different doses and schedules indicated in panel (A) Body weight during treatment. As shown in panel B, this drug was efficacious but mild, demonstrating that B-luc-GFP HT-29 colorectal orthotopic model could provide a powerful preclinical model for in vivo evaluation of DS-8201. Values are expressed as mean ± SEM. *p<0.05, ***p<0.01, ****p<0.001.
    Orthotopic Colorectal Cancer Model: B-Tg(Luc) RKO in B-NDG Mice
    Establishment of orthotopic colorectal cancer model
    Establishment of orthotopic colorectal cancer model
    Establishment of orthotopic colorectal cancer model.
    The colon of B-NDG mice were inoculated with B-Tg(Luc) RKO cell suspension (5×105, 1×106), and the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased and body weight of mice had modest weight loss over time. This indicates that the cell line was successfully constructed as an orthotopic tumor model. H&E staining showed that tumors metastasized in the liver, small intestine and pancreas.
    Orthotopic Colorectal Cancer Model: B-CMV-Luc LOVO#3-F03 + B-NDG mice (orthotopic)
    Study ID: Groups Animal strain Sex Cell line The amount of
    cells Inoculated    		 The Volume
    of cells    		 Animal number Remarks
    24P071544 G1 B-NDG mice F B-CMV-Luc LOVO 1×106 0.04ml/mice 6 Orthotopic, Cells mixed with Matrigel;1:1
    G2 B-NDG mice F B-CMV-Luc LOVO 2×106 0.04ml/mice 6 Orthotopic, Cells mixed with Matrigel;1:1
    G3 B-NDG mice F B-CMV-Luc LOVO 5×106 0.1ml/mice 6 Subcutaneously on the right flank
    Cells mixed with Matrigel;1:1
    B-CMV-Luc LOVO img
    Establishment of orthotopic colorectal cancer model.
    The B-NDG mice (F, n=6) were inoculated with B-CMV-Luc LOVO cell suspension (1×10⁶, 2×10⁶, 5×10⁶). And the body weight and tumor fluorescence signal of the mice were recorded weekly. The results showed that the fluorescence signal gradually increased. This indicates that the cell line was successfully constructed as an orthotopic tumor model.