Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hIgE/hFCER1A mice. Exons 1-6 of mouse Ige gene that encode the full-length protein were replaced by human IGHE exons 1-6 in B-hIgE/hFCER1A mice. Exons 1-5 of mouse Fcer1a gene that encode the extracellular domain were replaced by human FCER1A exons 3-7 in B-hIgE/hFCER1A mice.
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Protein expression analysis
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Species-specific IgE protein expression analysis in wild-type and humanized B-hIgE/hFCER1A mice. Following LPS stimulation in vivo, bone marrow-derived basophilic granulocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hIgE/hFCER1A (H/H) mice and analyzed by flow cytometry using species-specific anti-IgE antibodies. Murine IgE protein was detected in wild-type mice, while human IgE protein was detected in B-hIgE/hFCER1A mice.
Species–specific FCER1A protein expression analysis in wild-type and humanized B-hIgE/hFCER1A mice. Bone marrow-derived basophilic granulocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hIgE/hFCER1A (H/H) mice and analyzed by flow cytometry using species-specific anti-FCER1A antibodies. Murine FCER1A protein was detected in wild-type mice, while human FCER1A protein was detected in B-hIgE/hFCER1A mice.
