Basic Information
-
Targeting Strategy
-
Exons 2-7 of the mouse Cd40 gene, which encodes the extracellular region, were replaced by human CD40 exons 2-7 in B-hCD40/hCD40L mice. Exons 2-5 of the mouse Cd40l gene, which encodes the extracellular region, was replaced by human CD40L exons 2-5 in B-hCD40/hCD40L mice.
-
mRNA Expression
-
Species-specific analysis of CD40/CD40L gene expression in wild-type C57BL/6 mice and B-hCD40/CD40L mice by RT-PCR. Mouse CD40/CD40L mRNA was detectable in splenocytes of wild-type C57BL/6 mice (+/+). Human CD40/CD40L mRNA was detectable only in homozygous B-hCD40/CD40L (H/H) mice.
-
Surface Expression in Splenocytes
-
Species-specific CD40 expression in homozygous B-hCD40/hCD40L mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD40/hCD40L mice (H/H) stimulated with anti-CD3ε in vivo (7.5 μg/mice, stimulation for 24 hours, i.p.), and analyzed by flow cytometry with species-specific anti-CD40 antibody. Mouse CD40 was detectable in wild-type mice. Human CD40 was exclusively detectable in homozygous B-hCD40/hCD40L mice.
-
Surface Expression in Thymocytes
-
Species-specific CD40L expression in homozygous B-hCD40/hCD40L mice by flow cytometry. Thymocytes were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hCD40/hCD40L mice (H/H) and stimulated with PMA & Ionomycin, then analyzed by flow cytometry with species-specific anti-CD40L antibody. Mouse CD40L was detectable in wild-type mice. Human CD40L was exclusively detectable in homozygous B-hCD40/hCD40L mice.
