Basic Information

Strain Name
C57BL/6-Ncr1tm1NCR1/Bcgen
Common Name
B-hNKP46 mice
Background
C57BL/6J
Catalog Number
111946
Aliases
CD335, LY94, NK-p46, NKP46
NCBI Gene ID

Gene Targeting Strategy

Gene targeting strategy for B-hNKP46 mice. The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice.

Protein Expression Analysis

Species-specific NKP46 expression analysis in wild-type and humanized B-hNKP46 mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and heterozygous B-hNKP46 (H/+) mice and analyzed by flow cytometry using species-specific NKP46 antibodies. Mouse NKP46 was detectable in wild-type and heterozygous B-hNKP46 mice, while human NKP46 protein was exclusively detected in heterozygous B-hNKP46 mice.

Species-specific NKP46 expression analysis in wild-type and humanized B-hNKP46 mice. Blood was collected from wild-type C57BL/6 (+/+) and heterozygous B-hNKP46 (H/+) mice and analyzed by flow cytometry using species-specific NKP46 antibodies. Mouse NKP46 was detectable in wild-type and heterozygous B-hNKP46 mice, while human NKP46 protein was exclusively detected in heterozygous B-hNKP46 mice.

Summary

Protein expression analysis:

Human NKP46 was exclusively detectable in heterozygous B-hNKP46 mice but not wild-type mice, and mouse NKP46 was detectable in wild-type mice and heterozygous B-hNKP46 mice.