Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hNKP46 mice. The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice.
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Protein expression analysis
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Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 and homozygous B-hNKP46 (H/H) mice, and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice.
Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice by flow cytometry. Blood was collected from wild-type C57BL/6 and homozygous B-hNKP46 (H/H) mice, and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice.
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Analysis of leukocytes cell subpopulation in spleen
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Analysis of spleen leukocyte subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in spleen. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in spleen
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Analysis of spleen T cell subpopulations by FACS. Splenocytes were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in spleen. Values are expressed as mean ± SEM.
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Analysis of leukocytes cell subpopulation in lymph node
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Analysis of lymph node leukocyte subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the leukocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of T cells, B cells and NK cells in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in lymph node. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in lymph node
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Analysis of lymph node T cell subpopulations by FACS. Leukocytes were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in lymph node. Values are expressed as mean ± SEM.
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Analysis of leukocytes cell subpopulation in blood
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Analysis of blood leukocyte subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these cell types in blood. Values are expressed as mean ± SEM.
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Analysis of T cell subpopulation in blood
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Analysis of blood T cell subpopulations by FACS. Blood were isolated from female C57BL/6 and homozygous B-hNKP46 mice (n=3, 6-week-old). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live CD45+ cells were gated for CD3+ T cell population and used for further analysis as indicated here. B. Results of FACS analysis. The percent of CD8+ T cells, CD4+ T cells, and Tregs in homozygous B-hNKP46 mice were similar to those in the C57BL/6 mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation or distribution of these T cell subtypes in blood. Values are expressed as mean ± SEM.
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In vivo efficacy of an anti-human NKP46-based antibody
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Antitumor activity of anti-human NKP46-based Ab in B-hNKP46 mice. (A) Anti-human NKP46-based Ab inhibited MC38 tumor growth in B-hNKP46 mice. Murine colon cancer MC38 cells (5E5) were subcutaneously implanted into B-hNKP46 mice (female, 7-8 week-old, n=7). Mice were grouped when tumor volume reached approximately 100 mm3, at which time they were treated with anti-human NKP46-based Ab. (B) Body weight changes during treatment. As shown in panel A, anti-human NKP46-based Ab was efficacious in controlling tumor growth in B-hNKP46 mice. Values are expressed as mean ± SEM. (All antibodies were provided by the clients).
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Summary
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Protein expression analysis:
Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice but not wild-type mice, and mouse NKP46 was detectable in wild-type mice.
Leukocytes profile:
Percentages of T, B, NK cells, monocyte/macrophages, and DC were similar in homozygous B-hNKP46 mice and wild-type mice, demonstrating that introduction of hNKP46 in place of its mouse counterpart does not change the overall development, differentiation, or distribution of these cell types in spleen, lymph node leukocytes and blood.
In vivo efficacious：
Anti-human NKP46-based Ab were efficacious in controlling tumor growth in B-hNKP46 mice.
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Posters
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Humanized NKP46 mouse models for testing novel NK cell-based immunotherapies
