Basic Information
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Gene Targeting Strategy
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Gene targeting strategy for B-hNKP46 mice. The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice.
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Protein Expression Analysis
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Species-specific NKP46 expression analysis in wild-type and humanized B-hNKP46 mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and heterozygous B-hNKP46 (H/+) mice and analyzed by flow cytometry using species-specific NKP46 antibodies. Mouse NKP46 was detectable in wild-type and heterozygous B-hNKP46 mice, while human NKP46 protein was exclusively detected in heterozygous B-hNKP46 mice.
Species-specific NKP46 expression analysis in wild-type and humanized B-hNKP46 mice. Blood was collected from wild-type C57BL/6 (+/+) and heterozygous B-hNKP46 (H/+) mice and analyzed by flow cytometry using species-specific NKP46 antibodies. Mouse NKP46 was detectable in wild-type and heterozygous B-hNKP46 mice, while human NKP46 protein was exclusively detected in heterozygous B-hNKP46 mice.
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Summary
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Protein expression analysis:
Human NKP46 was exclusively detectable in heterozygous B-hNKP46 mice but not wild-type mice, and mouse NKP46 was detectable in wild-type mice and heterozygous B-hNKP46 mice.
Species-specific NKP46 expression analysis in wild-type and humanized B-hNKP46 mice. Splenocytes were collected from wild-type C57BL/6 (+/+) and heterozygous B-hNKP46 (H/+) mice and analyzed by flow cytometry using species-specific NKP46 antibodies. Mouse NKP46 was detectable in wild-type and heterozygous B-hNKP46 mice, while human NKP46 protein was exclusively detected in heterozygous B-hNKP46 mice.
