Basic Information
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Targeting Strategy
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Gene targeting strategy for B-hPD-L1/hLAG3 mice. The exon 3 of mouse Pdl1 gene that encodes the extracellular domain was replaced by human PD-L1 exon 3 in B-hPD-L1/hLAG3 mice. The exons 2-3 of mouse Lag3 gene that encode the extracellular domain were replaced by human LAG3 exons 2-3 in B-hPD-L1/hLAG3 mice.
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Details
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Phenotype
Protein Expression Analysis
Strain specific PD-L1 and LAG3 expression analysis in homozygous B-hPD-L1/hLAG3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hLAG3 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-LAG3 antibody. Mouse PD-L1 was detectable in WT mice, mouse LAG3 was detectable in WT and homozygous B-hLAG3 mice due to the anti-mouse LAG3 antibody cross-reacts with human LAG3. Human PD-L1 and LAG3 were exclusively detectable in homozygous hPD-L1/hLAG3 but not WT mice.
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Publications
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References
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- J Immunol Methods. 2017 Aug;447:47-51. doi: 10.1016/j.jim.2017.04.006. Epub 2017 Apr 21.
- Proc Natl Acad Sci U S A. 1997 May 27;94(11):5744-9.
- Nat Immunol. 2001 Dec;2(12):1109-16.
- Immunity. 2007 Mar;26(3):311-21.