B-hPD-L1/hLAG3 mice

Basic Information

Targeting Strategy

Gene targeting strategy for B-hPD-L1/hLAG3 mice. The exon 3 of mouse Pdl1 gene that  encodes the extracellular domain was replaced by human PD-L1 exon 3 in B-hPD-L1/hLAG3 mice. The exons 2-3 of mouse Lag3 gene that  encode the extracellular domain were replaced by human LAG3 exons 2-3 in B-hPD-L1/hLAG3 mice.

Details

Phenotype

Protein Expression Analysis

Strain specific PD-L1 and LAG3 expression analysis in homozygous B-hPD-L1/hLAG3 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-L1/hLAG3 (H/H) mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-LAG3 antibody. Mouse PD-L1 was detectable in WT mice, mouse LAG3 was detectable in WT and homozygous B-hLAG3 mice due to the anti-mouse LAG3 antibody cross-reacts with human LAG3. Human PD-L1 and LAG3 were exclusively detectable in homozygous hPD-L1/hLAG3 but not WT mice.

Publications

Jiang H, Ni H, Zhang P, Guo X, Wu M, Shen H, Wang J, Wu W, Wu Z, Ding J, Tang R, Zhou S, Chen B, Yu M, Jing H, Liu J. PD-L1/LAG-3 bispecific antibody enhances tumor-specific immunity. Oncoimmunology. 2021 Jun 24;10(1):1943180. doi: 10.1080/2162402X.2021.1943180. PMID: 34239776; PMCID: PMC8237984.

References

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