Basic Information

Strain Name
C57BL/6-Erbb2tm1(ERBB2)/Bcgen
Common Name
B-hHER2 mice
Background
C57BL/6
Catalog number
110812
Aliases
ERBB2, CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1, VSCN2
NCBI Gene ID

mRNA expression analysis

Species-specific HER2 gene expression analysis in wild type and humanized B-hHER2 mice. Murine Her2 mRNA was detected in the colon of wild-type (+/+) and heterozygous B-hHER2 (H/+) mice by RT-PCR. Human HER2 mRNA was exclusively detected in heterozygous B-hHER2 (H/+) mice compared to wild-type mice.

IHC analysis of HER2 expression

Immunohistochemical (IHC) analysis of HER2 expression in humanized B-hHER2 mice. Mammary gland, colon and stomach tissues were collected from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice and analyzed by IHC using an anti-Her2 antibody. HER2 was detected in wild-type and homozygous B-hHER2 mice due to the cross-reactivity of the antibody. The arrow indicates tissue cells with positive HER2 staining (brown).

Analysis of splenic leukocyte subpopulations in B-hHER2 mice

Analysis of splenic leukocyte subpopulations in humanized B-hHER2 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9-week-old), and analyzed by flow cytometry to assess splenic leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45 and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of splenic leukocytes. Values are expressed as mean ± SEM.

Analysis of splenic T cell subpopulations in B-hHER2 mice

Analysis of splenic T cell subpopulations in humanized B-hHER2 mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9 week-old), and analyzed by flow cytometry to assess splenic T cell subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on CD3 and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells and Tregs in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of splenic T cell subtypes. Values are expressed as mean ± SEM.

Analysis of lymph node leukocyte subpopulations in B-hHER2 mice

Analysis of lymph node leukocyte subpopulations in humanized B-hHER2 mice. Leukocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9 week-old), and analyzed by flow cytometry to assess lymph node leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45 and used for further analysis as indicated. (B) Percent of T cells, B cells and NK cells in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of lymph node leukocytes. Values are expressed as mean ± SEM.

Analysis of lymph node T cell subpopulations in B-hHER2 mice

Analysis of lymph node T cell subpopulations in humanized B-hHER2 mice. Leukocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9 week-old), and analyzed by flow cytometry to assess lymph node T cell subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on CD3 and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells and Tregs in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of lymph node T cell subtypes. Values are expressed as mean ± SEM.

Analysis of blood leukocyte subpopulations in B-hHER2 mice

Analysis of blood leukocyte subpopulations in humanized B-hHER2 mice. Blood cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9 week-old), and analyzed by flow cytometry to assess blood leukocyte subpopulations. (A) Representative flow cytometry plots. Single live cells were gated on CD45 and used for further analysis as indicated. (B) Percent of T cells, B cells, NK cells, dendritic cells, granulocytes, monocytes and macrophages in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of blood leukocytes. Values are expressed as mean ± SEM.

Analysis of blood T cell subpopulations in B-hHER2 mice

Analysis of blood T cell subpopulations in humanized B-hHER2 mice. Blood cells were isolated from wild-type C57BL/6 (+/+) and homozygous B-hHER2 (H/H) mice (female, n=3, 9 week-old), and analyzed by flow cytometry to assess blood T cell subpopulations. (A) Representative flow cytometry plots. Single live CD45+ cells were gated on CD3 and used for further analysis as indicated. (B) Percent of CD8+ T cells, CD4+ T cells and Tregs in homozygous B-hHER2 mice were similar to those in wild-type mice, demonstrating that introduction of hHER2 in place of its mouse counterpart does not change the overall development, differentiation or distribution of blood T cell subtypes. Values are expressed as mean ± SEM.