C57BL/6N-Sμtm1(IGHA1)BcgenGt(ROSA)26Sortm1(CD11b-FCAR)Bcgen/Bcgen • 112799
| Product name | B-hIGHA1/hCD89 mice |
|---|---|
| Catalog number | 112799 |
| Strain name | C57BL/6N-Sμtm1(IGHA1)BcgenGt(ROSA)26Sortm1(CD11b-FCAR)Bcgen/Bcgen |
| Strain background | C57BL/6N |
| Aliases | IGHA1 (IgA1);CD89 (FcalphaRI; CTB-61M7.2, FCAR, Fc fragment of IgA receptor) |
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Protein expression analysis in T, B cells
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was mildly detectable in T and B cells in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in serum
Strain specific IgA expression analysis in homozygous B-hIGHA1/hCD89 mice by ELISA. Serum were collected from 8-weeks wild-type mice C57BL/6 mice (+/+) and 8-weeks homozygous B-hIGHA1/hCD89 mice (H/H, H/+ or H/H, H/H), and analyzed by ELISA with species-specific IgA ELISA kit. hIgA was exclusively detectable in homozygous mice, but soluble IgA levels were higher in B-hIGHA1/hCD89 mice (H/H, H/+) compared to that in B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in NK cells and DCs 
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in NK cells and DCs in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in monocytes and macrophagesCD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in monocytes and macrophages in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in neutrophils
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in neutrophils in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in spleen
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was mainly detectable in NK cells, DCs, monocytes, macrophages and neutrophils in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Analysis of leukocytes cell subpopulation in spleen
Analysis of spleen leukocyte subpopulations by flow cytometry. Splenocytes were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H). Flow cytometry analysis of the splenocytes was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of B cells was decreased and T cells increased in B-hIGHA1/hCD89 mice accompanied with a smaller spleen compared to that in C57BL/6 mice. Values are expressed as mean ± SD.
Protein expression analysis in T, B cells
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in T and B cells in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in NK cells and DCs 
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in NK cells and DCs in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in monocytes and macrophages
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in monocytes and macrophages in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in neutrophils
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in neutrophils in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Protein expression analysis in Bone marrow
CD89 protein expression analysis in heterozygous and homozygous B-hIGHA1/hCD89 mice by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H), and then analyzed by flow cytometry with anti-hCD89 antibody. hCD89 was detectable in T, B, NK cells, DCs, monocytes, macrophages and neutrophils in B-hIGHA1/hCD89 mice (H/H, H/+) and B-hIGHA1/hCD89 mice (H/H, H/H).
Analysis of leukocytes cell subpopulation in bone marrow
Analysis of bone marrow leukocyte subpopulations by flow cytometry. Bone marrow cells were collected from wild-type C57BL/6 mice (+/+), heterozygous B-hIGHA1/hCD89 mice (H/H, H/+) and homozygous B-hIGHA1/hCD89 mice (H/H, H/H). Flow cytometry analysis of the bone marrow cells was performed to assess leukocyte subpopulations. A. Representative FACS plots. Single live cells were gated for the CD45+ population and used for further analysis as indicated here. B. Results of FACS analysis. Percent of B cells was decreased in B-hIGHA1/hCD89 mice compared to that in C57BL/6 mice. Values are expressed as mean ± SD.
Analysis of blood and urine
Blood and urine analysis: Four 30-week-old male B-hIGHA1/hCD89 mice (H/H,H/H) and C57BL/6N were selected to assess the blood and urine indicators. The results showed that the levels of creatinine (CREA) and urine protein in the urine, as well as blood urea nitrogen (UREA) and creatinine (CREA), did not show significant differences compared to wild-type mice.
Analysis of kidney tissue sections 
Analysis of kidney tissue sections: Four 30-week-old male B-hIGHA1/hCD89 mice(H/H,H/H) and C57BL/6N were selected for kidney tissue section immunohistochemical staining analysis. The results showed a slight increase in CD11b staining in the B-hIGHA1/hCD89 mice(H/H,H/H), while IgA and C3 staining showed no significant differences between B-hIGHA1/hCD89 mice(H/H,H/H) and C57BL/6N.
