• 321851
| Product name | B-hCCR4-luc EL4 |
|---|---|
| Catalog number | 321851 |
| Strain background | C57BL/6 |
| Aliases | CCR4, CC-CKR-4, CD194, CKR4, CMKBR4, ChemR13, HGCN:14099, K5-5 |
| Tissue | T lymphocyte |
| Disease | Lymphoma |
| Species | Mouse |
| Application | B-hCCR4-luc EL4 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe mouse Ccr4 gene was replaced by human CCR4 coding sequence and the luciferase sequence in B-hCCR4-luc EL4 cells. Human CCR4 is highly expressed on the surface of B-hCCR4-luc EL4 cells.
Targeting strategyGene targeting strategy for B-hCCR4-luc EL4 cells.The exogenous promoter and human CCR4 coding sequence and the luciferase sequence were inserted to replace part of murine exon 2. The insertion disrupts the endogenous murine Ccr4 gene, resulting in a non-functional transcript.
Protein expression analysis
CCR4 expression analysis in B-hCCR4-luc EL4 cells by flow cytometry. Single cell suspensions from wild-type EL4 and B-hCCR4-luc EL4 cultures were stained with species-specific anti-CCR4 antibody. Human CCR4-luc was detected on the surface of B-hCCR4-luc EL4 cells, but not on the surface of wild-type EL4 cells. The clones 3-F10 and 3-F11 of B-hCCR4-luc EL4 cells were used for in vivo tumor growth assays.
Protein expression analysis of tumor cells
B-hCCR4-luc EL4 cells were subcutaneously transplanted into C57BL/6 mice (n=5). At the end of the experiment, tumor cells were harvested and assessed for human CCR4 expression by flow cytometry. As shown, human CCR4 was highly expressed on the surface of tumor cells. Therefore, B-hCCR4-luc EL4 cells can be used for in vivo efficacy studies of CCR4 therapeutics.
The luminescence signal intensity analysis
Tumor growth curve & Body weight changes
Protein expression analysis of tumor cells
Tumor growth and in vivo imaging of B-hCCR4-luc EL4 cells. B-hCCR4-luc EL4 cells (2x105) were injected into the tail vein of wild-type C57BL/6 mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-hCCR4-luc EL4 cells can be used for in vivo efficacy evaluation.
