Mouse Abcb4 mRNA expression in wild-type BALB/C mice and B-Abcb4 KO mice(C) by RT-PCR. Liver and lung RNA were isolated from wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Abcb4 primers. Mouse Abcb4 mRNA was detectable only in wild-type BALB/C mice, but not in B-Abcb4 KO mice(C) (-/-).

H&E staining of B-Abcb4 KO mice(C). Liver tissues from BALB/C mice and B-Abcb4 KO mice(C) (n=3, Male, 6 week-old; n=2, Female, 6 week-old) were collected and analyzed for H&E staining. There were no abnormal changes in the liver of wild-type BALB/C mice, but in the liver of B-Abcb4 KO mice, proliferation of intrahepatic bile ducts, thickening of the duct walls, and a small amount of inflammatory cell infiltration could be observed. (a) Bile ducts (b) Inflammatory 

Sirius red staining of B-Abcb4 KO mice(C). (A) Representative  images of Sirius red staining showing liver fibrosis in wild-type BALB/C mice (+/+) and B-Abcb4 KO mice(C) (-/-) (n=3, Male, 6 week-old; n=2, Female, 6 week-old). (B) Statistic data of Sirius red  staining. Values are expressed as mean ± SEM. Significance was determined by two-way ANOVA test.  *P < 0.05, **P < 0.01, ***P < 0.001. 

Biochemical test of B-Abcb4 KO mice(C). Values are expressed as mean ± SEM. N=6 mice per group. Significance was determined by two-way ANOVA test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.