• 310686
| Product name | B-hPD-L1 LLC1 |
|---|---|
| Catalog number | 310686 |
| Strain background | C57BL/6 |
| Aliases | CD274, PDCD1L1 |
| Tissue | Lung |
| Disease | Lewis lung carcinoma |
| Species | Mouse |
| Application | B-hPD-L1 LLC1 cells have the capability to establish tumors in vivo and can be used for efficacy studies. |
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DescriptionThe mouse Pdl1 gene was replaced by human PD-L1 coding sequence in B-hPD-L1 LLC1 cells. Human PD-L1 is highly expressed on the surface of B-hPD-L1 LLC1 cells.
Targeting strategyThe exogenous promoter and human PD-L1 coding sequence was inserted to replace part of murine exon 3. The insertion disrupts the endogenous murine Pdl1 gene, resulting in a non-functional transcript.
Protein expression analysis
Tumor growth curve & Body weight changes
Subcutaneous homograft tumor growth of B-hPD-L1 LLC1 cells. B-hPD-L1 LLC1 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B) Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hPD-L1 LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.
Tumor growth curve of individual mice
Subcutaneous homograft tumor growth of B-hPD-L1 LLC1 cells. B-hPD-L1 LLC1 cells (2x105) were subcutaneously implanted into C57BL/6 mice (female, 7-week-old, n=5). As shown in panel, B-hPD-L1 LLC1 cells were able to establish tumors in vivo and can be used for efficacy studies.
