This B-Luc-EGFP MV-4-11 cell line expresses firefly luciferase as a marker of MV-4-11 cells. Luminescence could be detected in B-Luc-EGFP MV-4-11 cells.

Gene targeting strategy for B-Luc-EGFP MV-4-11 cells. The exogenous promoter and luciferase-EGFP were inserted into the targeting vector used in the lentivirus system.

Luminescence signal intensity of B-Luc-EGFP MV-4-11 cells. Luminescence intensity was measured using the Bright-GloTM luciferase Assay. B-Luc-EGFP MV-4-11 cells have a strong luminescence signal. EGFP was detected in B-Luc-EGFP MV-4-11 cells. The 1-D02 clone of B-Luc-EGFP MV-4-11 cells can be used in vivo experiments.

Tumor growth and in vivo imaging of B-Luc-EGFP MV-4-11 cells. B-Luc-EGFP MV-4-11 cells (1x10⁶) were injected into the tail vein of wild-type B-NDG mice. Signal intensity and body weight were measured twice a week. (A) Imaging was performed twice a week. (B) Body weight (Mean ± SEM). B-Luc-EGFP MV-4-11 cells can be used for in vivo efficacy evaluation.