B-hAFP mice

C57BL/6N-Afptm1(AFP)Bcgen/Bcgen • 111622

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B-hAFP mice

Catalog Number: 111622
Strain Name: C57BL/6N-Afptm1(AFP)Bcgen/Bcgen
Strain Background: C57BL/6N
NCBI gene ID: 174 (Human)
Aliases: AFPD; FETA; HPAFP
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B-hAFP mice

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  • Targeting strategy
  • Phenotypic analysis

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      Targeting strategy

      Gene targeting strategy for B-hAFP mice. A CDS that encoded the full-length protein of human AFP were inserted into the mouse exon2.

      mRNA and protein expression analysis
      Strain specific analysis of AFP gene expression in wild-type mice and B-hAFP mice by RT-PCR. Mouse Afp mRNA was detectable in fetal liver of wild-type mice (+/+). Human AFP mRNA was detectable only in homozygous B-hAFP mice (H/H) but not in wild-type mice.Strain specific AFP expression analysis in homozygous B-hAFP mice by western blot. Fetal liver tissue were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hAFP mice (H/H), and analyzed by western blot with anti-hAFP antibody. Mouse AFP was detectable in wild-type mice. Human AFP was only detectable in homozygous B-hAFP mice but not in wild-type mice.
      * When publishing results obtained using this animal model, please acknowledge the source as follows: The animal model [B-hAFP mice] (Cat# 111622) was purchased from Biocytogen.