C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd27tm1(CD27)Bcgen/Bcgen • 120518
| Product name | B-hPD-1/hCD27 mice |
|---|---|
| Catalog number | 120518 |
| Strain name | C57BL/6-Pdcd1tm1(PDCD1)Bcgen Cd27tm1(CD27)Bcgen/Bcgen |
| Strain background | C57BL/6 |
| NCBI gene ID | 5133,939 (Human) |
| Aliases | CD279, PD-1, PD1, SLEB2, hPD-1, hPD-l, hSLE1; S152, S152. LPFS2, T14, TNFRSF7, Tp55 |
Protein Expression Analysis
Strain specific CD27 expression analysis in homozygous B-hPD-1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-CD27 antibody. Mouse CD27 was detectable in WT and B-hPD-1/hCD27 mice. Human CD27 were exclusively detectable in B-hPD-1/hCD27 mice but not WT mice. This might result from the cross-recognition of hCD27 by anti-mCD27 antibodies.
Strain specific PD-1 expression analysis in homozygous B-hPD-1/hCD27 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD27 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 antibody. Mouse PD-1 was exclusively detectable in WT mice. Human PD-1 were exclusively detectable in B-hPD-1/hCD27 mice but not WT mice.
