Basic Information

Common Name
B-hCD73 MC38
Aliases
CALJA, NT5E, E5NT, NT, NT5, NTE
Catalog Number
310728
Cell Type/ Disease
Colon Carcinoma
Species/ Strain
Mus musculus, C57BL/6
Notice
Biocytogen provides engineering service to generate the humanized tumor cell lines from customer’s own cell lines. Our humanized tumor cell lines are for pharmacology services ONLY.

Description

The mouse Nt5e gene was replaced by human NT5E coding sequence in B-hCD73 MC38 cells. Human CD73 is highly expressed on the surface of B-hCD73 MC38 cells.

Target strategy

The exogenous promoter and human NT5E coding sequence were inserted to replace part of murine exon 2 and all of exon 3. The insertion disrupts the endogenous murine Nt5e gene, resulting in a non-functional transcript.

Protein expression analysis

CD73 expression analysis in B-hCD73 MC38 cells by flow cytometry. Single cell suspensions from wild-type MC38 and B-hCD73 MC38 cultures were stained with species-specific anti-CD73 antibody. Human CD73 was detected on the surface of B-hCD73 MC38 cells but not wild-type MC38 cells. The 1-D02 clone of B-hCD73 MC38 cells was used for in vivo experiments.

Tumor growth curve

Subcutaneous homograft tumor growth of B-hCD73 MC38 cells. B-hCD73 MC38 cells (5×105) and wild-type MC38 cells (5×105) were subcutaneously implanted into C57BL/6N mice (female, 6-8-week-old, n=5). Tumor volume and body weight were measured twice a week. (A) Average tumor volume ± SEM. (B)  Body weight (Mean± SEM). Volume was expressed in mm3 using the formula: V=0.5 X long diameter X short diameter2. As shown in panel A, B-hCD73 MC38 cells were able to establish tumors in vivo and can be used for efficacy studies.

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