Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hIGF1R mice. The chimeric CDS that encodes human extracellular domain and mouse intracellular domain was inserted into exon 2 of mouse Igf1r gene in B-hIGF1R mice.
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mRNA expression analysis
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Species-specific IGF1R gene expression analysis in wild-type and humanized B-hIGF1R mice by RT-PCR. Mouse Igf1r mRNA was detected in brain tissue isolated from wild-type C57BL/6 (+/+) mice, while human IGF1R mRNA was detected in homozygous B-hIGF1R (H/H) mice.
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Protein expression analysis
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Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Kidney was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.
Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Brain was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.
Strain specific IGF1R expression analysis in homozygous B-hIGF1R mice by flow cytometry. Lung was collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by flow cytometry with anti-IGF1R antibody.IGF1R was detectable in wild-type mice and homozygous B-hIGF1R mice, as the antibody is crossly reactive with IGF1R in human and mice. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.
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Immunofluorescence analysis
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Immunofluorescence analysis of isolated brain microvessels from the wild-type mice and homozygous B-hIGF1R mice. Brain microvessels were collected from wild-type mice (+/+) and homozygous B-hIGF1R mice, and analyzed by immunofluorescence with anti-hIGF1R antibody. Human IGF1R was exclusively detectable in homozygous B-hIGF1R but not in wild-type mice.
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Summary
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mRNA expression analysis:
Human IGF1R was exclusively detectable in homozygous B-hIGF1R mice but not wild-type mice, and mouse Igf1r was detectable in wild-type mice.
Protein expression analysis:
Mouse IGF1R was detectable in wild-type mice, human IGF1R was exclusively detectable in homozygous B-hIGF1R mice but not in wild-type mice.
