Basic Information

Strain Name
C57BL/6-Il11ra1tm1(IL11RA)/Bcgen
Common Name
B-hIL11RA mice
Background
C57BL/6
Catalog Number
110879
Related Gene
interleukin 11 receptor, alpha chain 1
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hIL11RA mice.

The exons 2-11 of mouse Il11ra1 gene that encode the extracellular domain were replaced by human IL11RA exons 2-11 in B-hIL11RA mice.

mRNA expression analysis

Strain specific analysis of IL11RA gene expression in wild-type mice and B-hIL11RA mice by RT-PCR.

Mouse Il11ra1 mRNA was detectable in liver of wild-type mice (+/+). Human IL11RA mRNA was detectable only in homozygous B-hIL11RA but not in wild-type mice.

Protein expression analysis

Strain specific IL11RA expression analysis in homozygous B-hIL11RA mice by western blot.

Tissue was collected from wild-type C57BL/6 mice (+/+) and homozygous B-hIL11RA mice (H/H), and analyzed by western blot with anti-IL11RA antibody. Mouse IL11RA1 and human IL11RA were detectable in wild type mice and homozygous B-hIL11RA mice, as the antibody is crossly reactive with IL11RA in human and mice. Mice IL11RA1 and human IL11RA were not detectable in kidney, which might be because of low protein levels compared with liver.

Summary

mRNA expression

Mouse Il11ra1 mRNA was detectable in liver of wild-type mice. Human IL11RA mRNA was detectable only in homozygous B-hIL11RA mice.

Protein  expression

Mouse IL11RA1 was exclusively detectable in wild-type mice. Human IL11RA was exclusively detectable homozygous B-hIL11RA mice.