Basic Information
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Targeting strategy
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Gene targeting strategy for B-hTFF1 mice.
The exons 1~3 of mouse TFF1 gene that encodes the full-length protein was replaced by human TFF1 exons 1~3 in B-hTFF1 mice.
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Protein expression analysis
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Western blot analysis of TFF1 protein expression in homozygous B-hTFF1 mice. Stomach and colon lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hTFF1 mice (H/H), and then analyzed by western blot with anti-TFF1 antibody. 1 μg/μL total proteins were loaded for western blotting analysis by JESS. Human TFF1 was exclusively detected in stomach in homozygous B-hTFF1 mice.
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mRNA expression analysis
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Strain specific analysis of TFF1 mRNA expression in wild-type C57BL/6 mice and B-hTFF1 mice by RT-PCR. Stomach (A) and colon (B) RNA were isolated from wild-type C57BL/6 mice (+/+) and homozygous B-hTFF1 mice (H/H), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse or human TFF1 primers. Mouse Tff1 mRNA was only detectable in wild-type C57BL/6. Human TFF1 mRNA was exclusively detectable in homozygous B-hTFF1 mice.
