Basic Information
-
Gene targeting strategy
-
Gene targeting strategy for B-hCD98HC mice. The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98HC mice.
-
mRNA expression analysis
-
Species-specific CD98 gene expression analysis in wild-type and humanized B-hCD98HC mice by RT-PCR. Murine Cd98 mRNA was detected in kidney tissue isolated from wild-type (+/+) mice, while human CD98 mRNA was detected in homozygous B-hCD98HC (H/H) mice.
-
Protein expression analysis
-
Species-specific CD98 protein expression analysis in wild-type and humanized B-hCD98HC mice. Splenocytes were isolated from wild-type C57BL/6 (+/+) and homozygous B-hCD98HC (H/H) mice and analyzed by flow cytometry using species-specific anti-CD98 antibodies. Murine CD98 protein was detected in wild-type mice, while human CD98 protein was detected in B-hCD98HC mice.
