Basic Information

Strain Name
C57BL/6-Slc3a2tm1(SLC3A2)/Bcgen
Common Name
B-hCD98HC mice
Background
C57BL/6
Catalog number
110983
Aliases
SLC3A2, 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE, CD98 heavy chain
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hCD98HC mice.

The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98HC mice.

mRNA expression analysis

Strain specific analysis of CD98 gene expression in wild type (WT) mice and B-hCD98HC mice by RT-PCR.

Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).

Protein expression analysis

Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by flow cytometry.

Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC mice (H/H), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).

Summary

mRNA expression analysis:

Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).

Protein expression analysis:

Human CD98 was exclusively detectable on T cells and B cells of homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+), and mouse CD98 was detectable only in WT mice (+/+).