Basic Information
-
Targeting strategy
-
Gene targeting strategy for B-hCD98HC mice.
The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98HC mice.
-
mRNA expression analysis
-
Strain specific analysis of CD98 gene expression in wild type (WT) mice and B-hCD98HC mice by RT-PCR.
Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
-
Protein expression analysis
-
Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by flow cytometry.
Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC mice (H/H), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
-
Summary
-
mRNA expression analysis:
Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
Protein expression analysis:
Human CD98 was exclusively detectable on T cells and B cells of homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+), and mouse CD98 was detectable only in WT mice (+/+).
