Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hCD98HC mice. The exons 2-10 of mouse Cd98 gene that encode the extracellular domain were replaced by human CD98 exons 4-12 in B-hCD98HC mice.
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mRNA expression analysis
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Strain specific analysis of CD98 gene expression in wild type (WT) mice and B-hCD98HC mice by RT-PCR. Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
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Protein expression analysis
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Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by flow cytometry. Splenocytes were collected from wild type (WT) mice (+/+) and homozygous B-hCD98HC mice (H/H), and analyzed by flow cytometry with species-specific anti-CD98 antibody. Mouse CD98 was detectable in WT mice (+/+). Human CD98 was exclusively detectable in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
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Protein expression analysis in brain
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Strain specific CD98 expression analysis in homozygous B-hCD98HC mice by Immunofluorescence staining. Brain was collected from wild type (WT) mice and homozygous B-hCD98HC mice (female,8 week old) and processed into paraffin sections. The paraffin sections were stained using an species-specific anti-human CD98HC antibody (green). Brain tissues were co-stained with an anti-mouse CD31 antibody to visualize microvascular endothelial cells (red). Representative results of WT mice (A) and homozygous B-hCD98HC mice (B) are shown. The results indicated that human CD98 was exclusively detectable on brain microvascular endothelium of homozygous B-hCD98HC mice, but not in WT mice.
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Posters
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Development of a preclinical CD98HC mouse model for therapeutic studies
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Summary
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mRNA expression analysis:
Mouse Cd98 mRNA was detectable only in kidney of WT mice (+/+). Human CD98 mRNA was detectable only in homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+).
Protein expression analysis:
Human CD98 was exclusively detectable on T cells and B cells of homozygous B-hCD98HC mice (H/H) but not in WT mice (+/+), and mouse CD98 was detectable only in WT mice (+/+).
Human CD98 was exclusively detectable on brain microvascular endothelium of homozygous B-hCD98HC mice, but not in WT mice.
