Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hNKP46 mice(C). The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice(C).
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Protein expression analysis in spleen
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Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C).
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Protein expression analysis in T and B cells of spleen
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Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was not detectable in T and B cells of wild-type mice. Human NKP46 was not detectable in T and B cells of homozygous B-hNKP46 mice(C).
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Protein expression analysis in blood
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Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Blood cells were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C).
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Protein expression analysis in T and B cells of blood
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Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Blood cells were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was not detectable in T and B cells of wild-type mice. Human NKP46 was not detectable in T and B cells of homozygous B-hNKP46 mice(C).
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Summary
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Protein expression analysis:
Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C) but not wild-type mice, and mouse NKP46 was detectable in wild-type mice.
