Basic Information
-
Gene targeting strategy
-
Gene targeting strategy for B-hNKP46 mice(C). The exons 1~7 of mouse NKp46 gene that encode the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice(C).
-
Protein expression analysis in spleen
-
Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C).
-
Protein expression analysis in T and B cells of spleen
-
Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Splenocytes were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was not detectable in T and B cells of wild-type mice. Human NKP46 was not detectable in T and B cells of homozygous B-hNKP46 mice(C).
-
Protein expression analysis in blood
-
Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Blood cells were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was detectable in wild-type mice. Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C).
-
Protein expression analysis in T and B cells of blood
-
Strain specific NKP46 expression analysis in wild-type and B-hNKP46 mice(C) by flow cytometry. Blood cells were collected from BALB/c (+/+) and homozygous B-hNKP46 mice(C) (H/H), and analyzed by flow cytometry with species-specific NKP46 antibody. Mouse NKP46 was not detectable in T and B cells of wild-type mice. Human NKP46 was not detectable in T and B cells of homozygous B-hNKP46 mice(C).
-
Posters
-
Humanized NKP46 mouse models for testing novel NK cell-based immunotherapies
-
Summary
-
Protein expression analysis:
Human NKP46 was exclusively detectable in homozygous B-hNKP46 mice(C) but not wild-type mice, and mouse NKP46 was detectable in wild-type mice.
