Basic Information
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Gene targeting strategy
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Gene targeting strategy for B-hNKP46 mice(C). Exons 1~7 of the mouse NKp46 gene that encodes the extracellular domain were replaced by human NKP46 exons 1~7 in B-hNKP46 mice(C).
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Protein expression analysis
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Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(C). Splenocytes were isolated from wild-type BALB/c (+/+) and heterozygous B-hNKP46 mice(C) (H/+), and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous mice, while human NKP46 protein was only detected in B-hNKP46 mice(C).
Species-specific NKP46 protein expression analysis in wild-type and humanized B-hNKP46 mice(C). Lymphocytes were isolated from wild-type BALB/c (+/+) and heterozygous B-hNKP46 mice(C) (H/+), and analyzed by flow cytometry using species-specific anti-NKP46 antibodies. Mouse NKP46 protein was detected in wild-type and heterozygous mice, while human NKP46 protein was only detected in B-hNKP46 mice(C).
