Basic Information
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Targeting strategy
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Gene targeting strategy for B-hPD-1/hCD39 mice. The exon 2 of mouse Pd-1 gene that encodes the extracellular domain was replaced by human PD-1 exon 2 in B-hPD-1/hCD39 mice. The coding region sequence that encodes the extracellular domain was inserted followed mouse Entpd1 exon 2 in B-hPD-1/hCD39 mice.
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Protein expression analysis
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Strain specific PD-1 and CD39 expression analysis in homozygous B-hPD-1/hCD39 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD39 mice, and analyzed by flow cytometry with species-specific anti-PD-1 and anti-CD39 antibody. Mouse PD-1 and CD39 were detectable in WT mice. Human PD-1 and CD39 were exclusively detectable in homozygous B-hPD-1/hCD39 mice but not WT mice.
Strain specific PD-1 and CD39 expression analysis in homozygous B-hPD-1/hCD39 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD39 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 and anti-CD39 antibody. Mouse PD-1 and CD39 were detectable in WT mice. Human PD-1 and CD39 were exclusively detectable in homozygous B-hPD-1/hCD39 mice but not WT mice.
