Basic Information

Strain Name
C57BL/6-Pdcd1tm1(PDCD1)Entpd1tm3(ENTPD1)/Bcgen
Common Name
B-hPD-1/hCD39 mice
Background
C57BL/6
Catalog number
121179
NCBI Gene ID
Related Genes
PD-1 (Programmed death-1) ; CD39 (ENTPD1, ectonucleoside triphosphate diphosphohydrolase 1)

Targeting strategy

Gene targeting strategy for B-hPD-1/hCD39 mice. The exon 2 of mouse Pd-1 gene that  encodes the extracellular domain was replaced by human PD-1 exon 2 in B-hPD-1/hCD39 mice. The coding region sequence that encodes the extracellular domain was inserted followed mouse Entpd1 exon 2 in B-hPD-1/hCD39 mice.

Protein expression analysis

Strain specific PD-1 and CD39 expression analysis in homozygous B-hPD-1/hCD39 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD39 mice, and analyzed by flow cytometry with species-specific anti-PD-1 and anti-CD39 antibody. Mouse PD-1 and CD39 were detectable in WT mice. Human PD-1 and CD39 were exclusively detectable in homozygous B-hPD-1/hCD39 mice but not WT mice.

Strain specific PD-1 and CD39 expression analysis in homozygous B-hPD-1/hCD39 mice by flow cytometry. Splenocytes were collected from WT and homozygous B-hPD-1/hCD39 mice stimulated with anti-CD3ε in vivo, and analyzed by flow cytometry with species-specific anti-PD-1 and anti-CD39 antibody. Mouse PD-1 and CD39 were detectable in WT mice. Human PD-1 and CD39 were exclusively detectable in homozygous B-hPD-1/hCD39 mice but not WT mice.