Basic Information

Strain Name
C57BL/6N-Trem2tm1(TREM2)/Bcgen
Common Name
B-hTREM2 mice
Background
C57BL/6N
Catalog Number
111176
Related Gene
TREM2, PLOSL2, TREM-2, Trem2a, Trem2b, Trem2c
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTREM2 mice.

The exons 1~5 and 3` UTR of mouse Trem2 gene that encode the full-length protein were replaced by human TREM2 exons 1~5 and 3` UTR in B-hTREM2 mice.

mRNA expression analysis

Strain specific analysis of  TREM2 gene expression in wild-type mice and B-hTREM2 mice by RT-PCR.

Mouse Trem2 mRNA was detectable in wild-type mice (+/+). Human TREM2 mRNA was detectable only in homozygous B-hTREM2 mice(H/H) but not in wild-type mice.

Protein expression analysis

Strain specific TREM2 expression analysis in homozygous B-hTREM2 mice by flow cytometry.

Microglia were collected from wild-type mice (+/+) and homozygous B-hTREM2 mice(H/H), and analyzed by flow cytometry with anti-TREM2 antibody. TREM2 was detectable in microglia of wild-type mice and homozygous B-hTREM2 mice, as the antibody is crossly reactive with TREM2 in human and mice.

Strain specific TREM2 expression analysis in homozygous B-hTREM2 mice by flow cytometry.

MC38 cells were inoculated into wild-type C57BL/6(+/+) and homozygous B-hTREM2 mice(H/H). Tumor tissues were harvested when tumor volume reached approximately 1000 mm3, and analyzed by flow cytometry with anti-TREM2 antibody. TREM2 was detectable in tumor macrophage of wild-type mice and homozygous B-hTREM2 mice, as the antibody is crossly reactive with TREM2 in human and mice.

Strain specific TREM2 expression analysis in heterozygous B-hTREM2 mice by western blot.

Brain were collected from wild-type mice (+/+) and heterozygous B-hTREM2 mice (H/+), and analyzed by western blot with species-specific anti-hTREM2 antibody. Mouse TREM2 was not detectable in wild-type mice. Human TREM2 was exclusively detectable in heterozygous B-hTREM2 mice.

In vivo efficacy of anti-human TREM2 antibody

Antitumor activity of anti-human TREM2 antibody in B-hTREM2 mice. (A) Anti-human TREM2 antibody inhibited MC38 tumor growth in B-hTREM2 mice. Murine colon cancer MC38 cells were subcutaneously implanted into homozygous B-hTREM2 mice (male, 6-7 week-old, n=6). Mice were grouped when tumor volume reached approximately 100 mm3, and treated with anti-hTREM2 antibody at doses and schedules in panel A. (B) Body weight changes during treatment. As shown in panel A, anti-human TREM2 antibody (in house) were efficacious in controlling tumor growth in B-hTREM2 mice, demonstrating they provide a powerful preclinical model for in vivo evaluation of anti-human TREM2 antibody. Values are expressed as mean ± SEM.

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