Basic Information

Strain Name
C57BL/6-Trem2tm1(TREM2)/Bcgen
Common Name
B-hTREM2 mice
Background
C57BL/6
Catalog Number
111176
Related Gene
TREM2, PLOSL2, TREM-2, Trem2a, Trem2b, Trem2c
NCBI Gene ID

Targeting strategy

Gene targeting strategy for B-hTREM2 mice.

The exons 1~5 and 3` UTR of mouse Trem2 gene that encode the full-length protein were replaced by human TREM2 exons 1~5 and 3` UTR in B-hTREM2 mice.

Protein expression analysis

Strain specific TREM2 expression analysis in heterozygous B-hTREM2 mice by western blot.

Brain were collected from wild-type mice (+/+) and heterozygous B-hTREM2 mice (H/+), and analyzed by western blot with species-specific anti-hTREM2 antibody. Mouse TREM2 was not detectable in wild-type mice. Human TREM2 was exclusively detectable in heterozygous B-hTREM2 mice.

Summary

Protein expression analysis

Mouse TREM2 was not detectable in wild-type mice. Human TREM2 was exclusively detectable in heterozygous B-hTREM2 mice.