Basic Information

Strain Name
C57BL/6N-Fmr1tm1 Bcgen/Bcgen
Catalog number
112852
Common Name
B-Fmr1 KO mice
Background
C57BL/6N

Targeting strategy

Gene targeting strategy for B-Fmr1 KO mice.

The exons 3-17 of mouse Fmr1 gene were deleted in B-Fmr1 KO mice.

Protein expression analysis

Western blot analysis of FMRP protein expression in homozygous B-Fmr1 KO mice. Various tissue lysates were collected from wild-type C57BL/6 mice (+/+) and homozygous B-Fmr1 KO mice (-/Y), and then analyzed by western blot with anti-FMRP antibody. 40 μg total proteins were loaded for western blotting analysis. FMRP was not detectable in homozygous B-Fmr1 KO mice.

Open Field Test

Male B-Fmr1 KO mice show significant hyperactivity in the open field test. C57BL/6 mice and B-Fmr1 KO mice at 2 to 3 months of age were placed in an open field arena, and allowed to freely explore for 2 hours. The total movement distance (A), average speed (B), number of feces pellet (C), time spent in the periphery and center (D,E), and number of crossing in the periphery and center (F,G) were scored. Data presented as mean ± SEM were analyzed by T-test. n=10, *P < 0.05.

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