Basic Information

Strain Name
C57BL/6JNifdc-Mc4rtm1 Bcgen/Bcgen
Catalog number
112986
Common Name
B-Mc4r KO mice
Background
C57BL/6JNifdc
Application
This product is used for pharmacodynamics of diet-induced hepatic steatosis, hepatic fibrosis.

Gene editing strategy

Gene targeting strategy for B-Mc4r KO mice. 

The genome sequences of mouse Mc4r gene was depleted in B-Mc4r KO mice. As a result, mouse MC4R protein will be not expressed anymore.

mRNA expression analysis

Strain specific analysis of Mc4r mRNA expression in wild-type C57BL/6 mice and B-Mc4r KO mice by RT-PCR. Spinal cord RNA was isolated from wildtype C57BL/6 mice (+/+) and homozygous B-Mc4r KO mice (-/-), then cDNA libraries were synthesized by reverse transcription, followed by PCR with mouse Mc4r primers. Mouse Mc4r mRNA was detectable in wild-type C57BL/6 mice but not in B-Mc4r KO mice.

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