mRNA expression analysis
Strain specific analysis of ANTXR2 gene expression in wild-type C57BL/6 mice and B-hANTXR2 mice by RT-PCR. Mouse Antxr2 mRNA was detectable in wild-type C57BL/6 mice (+/+). Human ANTXR2 mRNA was detectable only in homozygous B-hANTXR2 mice (H/H), but not in wild-type mice.
Protein expression analysis
Strain specific ANTXR2 expression analysis in homozygous B-hANTXR2 mice by flow cytometry. Lung endothelial cells were collected from wild-type C57BL/6 mice (+/+) and homozygous B-hANTXR2 mice (H/H), and analyzed by flow cytometry with species-specific anti-hANTXR2 antibody. Human ANTXR2 was exclusively detectable in homozygous B-hANTXR2 but not in wild-type mice.
